Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report Date:
1987

Materials and methods

Principles of method if other than guideline:
Study conducted according to Bringmann and Kuehn
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Strain: DSM 50026
- Culture medium: AK-Medium according to DIN 38412 part 8 (draft)
- Temperature: 24°C
- Duration of growth: 7 +/-1 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
20°C
pH:
3.4 - 6.3
Nominal and measured concentrations:
- Concentrations tested (nominal): 7.81, 15.625, 31.25, 62.5, 125, 250, 500 and 1000 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
156.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
199.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
242.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

- Decrease of pH-value at test concentrations > 125 mg/L:

Conc. (mg/L) pH

7.81 6.3

15.625 6.2

31.25 6.3

62.5 6.1

125 6.0

250 4.3

500 3.8

1000 3.4

Applicant's summary and conclusion