kresoxim-methyl (ISO);methyl (E)-2-methoxyimino-[2-(o-tolyloxymethyl)phenyl]acetate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC guideline XI/681/86, draft 4

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

EC Number:

604-351-6

Cas Number:

143390-89-0

Molecular formula:

C18 H19 N O4

IUPAC Name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

Details on test material:

- Name of test material (as cited in study report): REG 242 009 techn.
- Physical state: Powder
- Analytical purity: 93.7% active ingredient
- Batch No.: N 36

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution was freshly prepared at the beginning of the test and before changing the test solution. 400 - 2000 µg/L substance were suspended directly in the test medium by stirring. By dilution of the stock solution with test medium the nominal concentrations were prepared.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age of parental stock: 2- 24 hours
- Feeding during test
- Food type: Scenedesmus subspicatus
- Amount: 0.22 mg COD at day 0-1, 0.25 mg COD at day 2-3, 0.35 mg at day 4-5, 0.43 mg at day 6-7 and 0.75 at day 8-ff.
- Frequency: daily

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

2.7 ± 0.5 mmol/L

Test temperature:

19.3 - 21.0 °C

pH:

7.1 - 7.9

Dissolved oxygen:

7.5 - 9.0 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control*, 0.01, 0.03, 0.1, 0.32, 1.0, 3.2, 10, 32, 100*, 320* µg/L
* = analytically analysed concentrations
Recovery rate of the analytically analysed concentrations: 94.4-99.1% (without daphnids, 0 h), 79.3-94.6% (without daphnids, 48/72 h), 70.7-99.8% (with daphnids, 48/72 h)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, total volume, fill volume: glass beakers covered with cups, 100 ml, 50 ml
- Renewal rate of test solution: each Monday, Wednesday, Friday
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: 0.02 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium M4 was prepared on the basis of an ultrapure, deionized water. The medium was aerated until saturated with oxygen, and was left to stand for 24 hours for stabilization.
- Alkalinity: 0.9 ± 0.1 mmol/L
- Ca/Mg ratio: ca. 4:1
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement:
- Temperature: Continuously in an extra vessel close to the test vessels
- pH and oxygen content: At the start of the test and at each change of test solution in the 48 or 72-hour-old solution in one parallel at each concentration

OTHER TEST CONDITIONS
- Photoperiod: day : night rhythm 16:8 hours
- Light intensity: about 5-6 µE/(m²s) in the range of 400 - 700 nm

EFFECT PARAMETERS MEASURED: Survival and reproduction, daily

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

32 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

100 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

other: LC0

Effect conc.:

100 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Time to first brood release or time to hatch: In the control the first young were observed at day 7. In the highest concentration tested, at which the daphnids produced young (100 µg/L), the first young were observed at day 9.