kresoxim-methyl (ISO);methyl (E)-2-methoxyimino-[2-(o-tolyloxymethyl)phenyl]acetate

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 165-4 (Laboratory Studies of Pesticide Accumulation in Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

EC Number:

604-351-6

Cas Number:

143390-89-0

Molecular formula:

C18 H19 N O4

IUPAC Name:

methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate

Details on test material:

- Name of test material (as cited in study report): BAS 490F
- Physical state: white solid
- Analytical purity: 99.6%
- Lot No.: 39/149-1
- Radiochemical purity: 97%
- Specific activity: 1117.4 MBq/mmol, 30.2 mCi/mmol adjusted to 10400 dpm/µg
- Locations of the label: Benzyl ring (B-label)

Radiolabelling:

yes

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: fish were randomly sampled (during exposure: 10 fish on Day 0, 14, 28, 5 fish on Day 3, 7, 21; during depuration: 3 fish on Day 1, 3, 7, 10, 14
- Sampling intervals/frequency for test medium samples: ca. 500 ml prior adding the fish, after 3, 7, 14, 21, 28 days during exposure period and on the first and last day of depuration; ca. 50 ml were taken on the other days of exposure
- Details on sampling and analysis of test organisms and test media samples:
- Sampling of test organisms: after sampling each fish was blotted dry, separated into viscara and fillet, weighted and stored <= 15 °C
- Extraction of radioactivity from fish samples: from viscera (4-15 g) and fillet (9-24 g) from Days 21 and 28
- Extraction of lipid: pooled samples of viscera and fillet from control fish taken at 0, 14, 28 days of the exposure period
- Extraction of tank water samples: ca. 300 ml for determination of the hydraulic stability of 14C BAS 490F on Days 0, 3, 7, 14, 21 and 28 of exposure

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Parkwood Trout Farm, Kent, UK
- Age at study initiation: ca. 1 year
- Length at study initiation: 5.1 ± 0.5 cm
- Feeding during test
- Food type: commercial trout pellets (BP Mainstream Fry Pellet No. 2; SDS Diets Services, Witham, Essex, UK)
- Amount: to repletion
- Frequency: twice daily

ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions: fiberglass tanks containing 300 L water (loading <1 fish per litre), temperature of 14 ± 1° C, aerated to give > 9.7 mg O2/L, dechlorinated tap water was renewed by a continuous flow system

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

14 d

Test conditions

Test temperature:

13- 14 °C

pH:

7.6 - 7.8

Dissolved oxygen:

9.8 - 10.1 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, total volume, fill volume: glass aquaria, ca.100 L, 100 L
- Aeration: >= 10 mg/O2/L
- Renewal rate of test solution (flow rate): 1440 L/vessel/day
- No. of organisms per vessel: ca. 50
- No. of vessels per concentration (replicates): 1
- No. of vessels per control: 1
- Biomass loading rate: < 4 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water (by addition of sodium thiosulphate and passage through activated carbon

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Feeding: twice daily to repletion

PRELIMINARY STUDY
- Reason for the preliminary study: to confirm the steady state
- Test concentration: 0.025 mg/L

Nominal and measured concentrations:

Nominal test concentration: 0.025 mg/L

Results and discussion

Lipid content

Lipid content 1

Lipid content:

4.02 %

Time point:

start of exposure

Remarks on result:

other: referring to total fish (viscera=5.95%; fillet=3.07%)

Lipid content 2

Lipid content:

4.48 %

Time point:

other: after 14 days

Remarks on result:

other: referring to total fish (viscera =8.43%; fillet=2.33)

Lipid content 3

Lipid content:

7.65 %

Time point:

end of exposure

Remarks on result:

other: refered to total fish (viscera=7.65%; fillet=2.34%)

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

220

Basis:

other: whole fish

Remarks on result:

other: mean BCF for 7-28 days

Remarks:

Conc.in environment / dose:25 µg/L

Bioaccumulation factor 2

Type:

BCF

Value:

430

Basis:

other: viscera

Remarks on result:

other: mean BCF for 7-28 days

Remarks:

Conc.in environment / dose:25 µg/L

Bioaccumulation factor 3

Type:

BCF

Value:

52

Basis:

edible fraction

Remarks on result:

other: mean BCF for 7-28 days

Remarks:

Conc.in environment / dose:25 µg/L

Depuration

Depuration 1

Elimination:

yes

Parameter:

other: DT50 (0-3 days) for total fish

Depuration time (DT):

0.37 d

Depuration 2

Elimination:

yes

Parameter:

other: DT50 (3-10days) for total fish

Depuration time (DT):

3.4 d

Details on kinetic parameters:

- Plateau during exposure: 5-6 µg equiv/g during 3-28 days of exposure
- Indication of biphasic kinetics: during depuration

Metabolites:

BAS 490F, BF 490-4, F2, BF 490-1, 242010, BF 490-9, BF 490-2