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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
other: U.S. EPA Pesticide Assessment Guideline FIFRA Subdivision E, 72-1
Qualifier:
according to guideline
Guideline:
other: ASTM Standard E729-88a
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
EC Number:
604-351-6
Cas Number:
143390-89-0
Molecular formula:
C18 H19 N O4
IUPAC Name:
methyl (2E)-2-methoxyimino-2-[2-[(2-methylphenoxy)methyl]phenyl]acetate
Details on test material:
- Name of test material (as cited in study report): BAS 490 F
- Physical state: light brown solid
- Analytical purity: 94.3% active ingredient
- Batch No.: N36
- Storage condition of test material: ambient temperature

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: each replicate
- Sampling time: at 0, 48, 96 h
- Sample storage conditions before analysis: no storage

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: One stock solution was prepared for each of the five concentrations. The first stock solution was prepared by dissolving BAS 490 F in dimethylformamide (DMF) at a concentration of 5.3 mg BAS 490 F/ml. The stock solution was inverted and stirred several times to aid solubilization of BAS 490 F. Aliquots of the stock solution were diluted with DMF to prepare four additional stock solutions at concentrations of 3.2, 1.9, 1.2 and 0.69 mg/ml. Stock solutions were prepared one time during the test period.
- Chemical name of vehicle: dimethylformamide (DMF)
- Concentration of vehicle in test medium (final test solution): 0.10 ml/L

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: Eggs from Troutlodge, Inc., Sumner, WA, USA, held in the culture facility of Wildlife International Ltd. until they achieved the proper size for testing.
- Age at study initiation: juveniles
- Length at study end (mean, range): 38 mm, 32 - 45 mm
- Weight at study end (mean, range): 0.64 g, 0.40 - 1.1 g
- Feeding during test: no

HOLDING CODITIONS
- Holding period: ca. 85 days
- Holding conditions:
- Water temperature 11.8 to 12.2°C
- pH: 7.8 to 8.0
- Oxygen content: 7.0 to 8.2 mg/L
- Type and amount of food: commercially-prepared diet supplied by Zeigler Brothers, Inc., Gardners, PA, USA.

ACCLIMATION
- Acclimation period: ca. 50 days
- Acclimation conditions: same as test
- Feeding during acclimation: no
- Health during holding and acclimation: the fish showed no signs of disease or stress

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
128 mg/L CaCO3
Test temperature:
11.3 - 12.0 °C
pH:
8.3 - 8.4
Dissolved oxygen:
9.2 - 9.9 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 65, 108, 180, 300, 500 µg a.i./L
Mean measured concentrations: -, 63, 104, 190, 314, 475 µg a.i./L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume, depth: Teflon-lined polyethylene aquaria, 25 L, 15 L, ca. 18 cm
- Type of flow-through: proportional diluter
- Renewal rate of test solution: approx. 6 volume additions of test water every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 0.071 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: moderately-hard well water obtained from a well 45 meters deep located on the Wildlife International Ltd. site. The well water was passed through a sand filter to remove particles greater than 25 µm and pumped into a 37800 L storage tank where the water was aerated. Prior to use, the water was filtered to remove microorganisms and particles.
- Alkalinity: 172 - 180 mg/L as CaCO2
- Conductivity: 310 -320 µmhos/cm
- Intervals of water quality measurement: temperature after 0 and 96 h, pH and dissolved oxygen after 0, 24, 48, 72, 96 h

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness with a 30 min transition period of low light intensity between changes in lightning
- Light intensity: ca. 260 lux

EFFECT PARAMETERS MEASURED: mortality after 2, 24, 48, 72, 96 hours

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations:, control, solvent control, 16, 54, 180, 600, 2000 µg a.i./L

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
104 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
190 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL = 163 -216 µg a.i./L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
104 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Details on results:
- Mortality of control: none
- Mortality of solvent control: none
- Behavioural abnormalities:
- 190 µg a.i./L treatment group: 1 lethargic fish after 24 h; 15 lethargic fish after 72 h; 3 lethargic fish after 96 h
- 314 µg a.i./L treatment group: 14 lethargic fish after 2 h; 3 lethargic fish after 24 h; 1 fish lying on bottom with little motion other than gill movement after 48 h, 1 lethargic fish after 72 and 96 h

Applicant's summary and conclusion