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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 Apr - 04 Oct 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
(July 1992)
Deviations:
yes
Remarks:
1) test substance concentration was not measured 2) mean length of fish (6.2 cm) was above the range specified in the TG (6.0 cm) 3) loading rate (1.08 g/L) slightly exceeded the limit specified in the TG (1 g/L)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Stock solution: two 10 mg/L test media batches were prepared by direct addition of Atmer 163 to dilution water and stirring for ca. 4 h
- Test solutions: one batch was used directly for the 1 mg/L test medium, the second batch was diluted with water to prepare the remaining test media
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Source: Brow Well Fisheries Limited, Skipton, North Yorkshire
- Length at study initiation: 6.2 cm (5.2 - 6.9 cm)
- Weight at study initiation: 2.1566 g (1.448 - 2.9107 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: ≥ 12 days
- Type and amount of food: a proprietary food (Trouw 00), quantities depening on the size of the fish >= 24 h prior to test initiation feeding was stopped
- Health during acclimation: cumulative mortality was < 5% over a seven day period preceding the test
- Density: appropriate to fish size
- Water in tank was replaced continuously
- Photoperiod: 16 h light / 8 h dark
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
67 - 78 as CaCO3 mg/L
Test temperature:
13.0 - 13.9 °C
pH:
7.2 - 8.2
Dissolved oxygen:
86 - 106 % air saturation
Salinity:
s
Nominal and measured concentrations:
nominal: control, 0.063, 0.125, 0.25, 0.5 and 1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquaria
- Type: closed; vessels were covered with perspex lids
- Material, size: glass, 15 L
- Renewal rate of test solution (semi-static conditions): renewal at 24 h intervals
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: ca. 1.08 g/L (7 fish each 2.157 g in 14 L test medium)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: mains dechlorinated water was used for the holding of stocks and for the toxicity tests
- Residual chlorine: 0.01 - 0.07 mg/L
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark


EFFECT PARAMETERS MEASURED:
- Four categories: no effects, mild toxic effects, severe toxic effects and death
- Intervals of observations: 24 h intervals (1, 24, 48, 72 and 96 h)


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.704 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
0.515 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.355 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: Behavioural abnormalities (e.g. swimming abnormally, increase cough frequency) were observed at a concentration of 0.5 mg/L after 24 h and at a concentration of 0.25 mg/L at the end of the test (96 h).
- Mortality of control: 0%
Reported statistics and error estimates:
Probit analysis after log10 transformation of the nominal concentrations.

Table 1: Toxic effects exhibited in the definitive test by O. mykiss exposed to Atmer 163

Nominal concentration [mg/L]

No. Of Fish

1 h

24 h

48 h

72 h

96 h

Control

7

0

0

0

0

0

0.063

7

0

0

0

0

0

0.125

7

0

0

0

0

0

0.25

7

0

0

0

0

0

0.5

7

0

0

0

3

7

1.0

7

0

1

7

7

7

Description of key information

LC50 (96 h): 0.355 mg/L (nominal) for Oncorhynchus mykiss (OECD 203)

Key value for chemical safety assessment

LC50 for freshwater fish:
0.355 mg/L

Additional information

The acute toxicity of Atmer 163 (CAS No. 97925-95-6) to Oncorhynchus mykiss was investigated in accordance to OECD 203 under GLP conditions (Mattock, 2001). In a semi-static test system fish were exposed to nominal concentrations of 0.063, 0.125, 0.25, 0.5 and 1 mg/L. No analytical monitoring of test substance concentrations was performed. Mortality was observed at the two highest test substance concentrations and a LC50 (96 h) of 0.355 mg/L was calculated.