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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 Apr - 04 Oct 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study with acceptable restrictions.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
1) test substance concentration was not measured; 2) after 24 h temperature in 4 vessels was outside the allowed range of ± 1°C, the variation was 1.1 to 1.3 °C; 3) ASTM medium was used
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Stock solution: 10 mg/L of stock test medium was prepared by direct addition of Atmer 163 to ASTM medium and stirring for ca. 4 h
- Test solutions: prepared by dilution of the 10 mg/L stock solution
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: laboratory culture at Covance (culture originally obtained from Sheffield University, UK)
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no feeding
- juveniles used were from the third brood onwards from adults
- only actively swimming individuals were used in test

CULTURING
- Medium: 500 mL ASTM standard hard water (see below: details on test conditions)
- Density: ca. 20 animals/L
- Feeding frequency: daily
- Type and amount of food: Chlorella vulgaris (ca. 5x10E5 cells/mL) plus seaweed extract as dietary supplement
- Water was renewed weekly
- Cultures were maintained up to a max. of 42 days
- New cultures were started with D. magna < 24 h

ACCLIMATION
- Acclimation period: 24 h
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
162 (0 h) - 196 mg/L (24 h) as CaCO3
Test temperature:
19.5 - 20.6 °C
pH:
7.7 - 8.0
Dissolved oxygen:
96 - 109% air saturation
Nominal and measured concentrations:
nominal: control, 0.065, 0.13, 0.25, 0.5 and 1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 150 mL glass crystallising dishes
- Renewal rate of test solution (semi-static conditions): one renewal after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 animals per 100 mL test solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: culturing and testing water was a standard hard water prepared according to the standards of the American Society for Testing an Materials (ASTM) => compounds are similar to OECD guideline 202 but in lower concentration
- Composition of ASTM:
=> NaHCO3: 192 mg/L
=> CaSO4 x 2H2O: 120 mg/L
=> MgSO4 x 7H2O: 246 mg/L
=> KCl: 8 mg/L
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 h of fluorescent lighting / 8 h dark

EFFECT PARAMETERS MEASURED:
- Effect parameter: immobilsation (swimming behaviour)
- Intervals of observations: after 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.85 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 0.71 - 1.02 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.59 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 0.5 - 0.7 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: 0%
Reported statistics and error estimates:
Probit analysis after log10 transformation of the nominal concentrations

Table 1: Immobilisation of Daphnia magna exposed to Atmer 163 over a 48 h period.

Nominal concentration [mg/L]

Number of Daphnia magna

Number immobilised

 

 

24 h

48 h

Control

20

0

0

0.065

20

0

0

0.13

20

0

0

0.25

20

0

0

0.50

20

1

6

1.0

20

14

19

Description of key information

EC50 (48 h): 0.59 mg/L (nominal) for Daphnia magna (OECD 202)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.59 mg/L

Additional information

The toxicity of Atmer 163 (CAS No. 97925-95-6) to aquatic invertebrates was investigated according to OECD 202 (GLP; Mattock, 2001). Daphnia magna was exposed under semi-static conditions to nominal test concentrations of 0.065, 0.13, 0.25, 0.5 and 1 mg/L. The mobility of D. magna was affected by the test substance. An EC50 of 0.59 mg/L was calculated based on mobility after an exposure period of 48 h.