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EC number: 308-208-6 | CAS number: 97925-95-6
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 of 27 mg/L, 95% confidence limits 22 - 33 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 13 mg/L.
Table 1: Oxygen consumption rates and percentage inhibition values after 3 hours contact time
Nominal concentration [mg/L]
Initial O2 reading
Measurement period [min]
Final O2 reading
O2 Consumption rates
 Increase in respiration rate as compared to controls
Observations made at 0 hours, 30 minutes and 3 hours showed that the control vessels contained a dark brown dispersion and the reference item vessels contained a dark brown dispersion with no undissolved reference item visible.
Observations made of the test item vessels at 0 hours prior to the addition of activated sewage sludge and synthetic sewage showed that the test concentration of 10 mg/L contained a clear colourless solution and the test concentration of 32 mg/L contained a very slightly cloudy dispersion with no undissolved test item visible. The test concentration of 100 mg/L contained a slightly cloudy dispersion with no undissolved test item visible and the test concentrations of 320 and 1000 mg/L contained cloudy dispersions with no undissolved test item visible. Observations made of the test item vessels after 30 minutes and 3 hours contact time showed that the test concentration of 10 mg/L contained a dark brown dispersion with no undissolved test item visible and the test concentrations of 32 and 100 mg/L contained dark brown dispersions with foam on the surface and no undissolved test item visible. The test concentrations of 320 and 1000 mg/L contained dark brown/grey dispersions with foam on the surface and no undissolved test item visible.
NOEC (3 h): 13 mg/L for activated sludge microorganisms (OECD 209)
One study investigated the effects of the test substance Atmer 163 (CAS No. 97925-95-6) to activated sludge microorganisms (Clarke, 2010). The test was performed according to the OECD Guideline 209 (GLP). Activated sewage sludge from a domestic sewage treatment plant was used as inoculum and exposed to nominal concentrations of 10, 32, 100, 320 and 1000 mg/L. The study resulted in an EC50 (3 h) of 27 mg/L (95% CL: 22 - 33 mg/L) based on the respiration of microorganisms in activated sewage sludge and in a NOEC (3 h) of 13 mg/L. It can be assumed that the test substance has toxic effects to microorganisms in activated sewage sludge.
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