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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: EEC Directive 92/32/EEC (December 1992) .
Deviations:
not specified
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Details on test organisms:
TEST ORGANISM
- Common name:Daphnia Magna
- Strain: Straus
- Source: originally supplied by the Institut National de Recherche Chimique Appliqu6e, France, then ultured and bred continuously in the Laboratory of Ecology of BASF AG in Ludwigshafen .
- Age at study initiation : 1 - 24 hours
- Feeding during test No


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no

Test conditions

Test temperature:
20.3 - 21,5 °C
pH:
7,9 - 8,1
Dissolved oxygen:
8,4 - 8,6 mg O2/L
Nominal and measured concentrations:
Nominal concentrations:
0, 12.5, 25, 50, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Fill volume: 10 ml
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
Illumination Artificial light, type warm white (e.g. OSRAM L58 W31) day:night rhythm = 16 : 8 h
- Light intensity: About 2 - 7 pE/(M2.s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Check of the study and recording (swimming ability) Visually after 0, 3, 6, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 12.5, 25, 50, 100 mg/L nominal
Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The EC50(24h) of the control substance potassium dichromate was 1,04 mg/I

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for the toxicity to Daphnia is greater than 100 mg/L
Executive summary:

The substance was tested to check its influence on the mobility to Daphnia Magna Straus according to EEC Directive 92/32/EEC (December 1992) equivalent or similar to OECD guideline 202. Four concentrations 12.5, 25, 50, 100 mg/L nominal were used.

After 48 hours no effect on the mobility of the Daphnia could be observed at each concentration. The EC50 is therefore greater than 100 mg/L.