1,4-bis(butylamino)anthraquinone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: EEC Directive 92/32/EEC (December 1992) .

Deviations:

not specified

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-bis(butylamino)anthraquinone

EC Number:

241-379-4

EC Name:

1,4-bis(butylamino)anthraquinone

Cas Number:

17354-14-2

Molecular formula:

C22H26N2O2

IUPAC Name:

1,4-bis(butylamino)anthraquinone

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Details on test organisms:

TEST ORGANISM
- Common name:Daphnia Magna
- Strain: Straus
- Source: originally supplied by the Institut National de Recherche Chimique Appliqu6e, France, then ultured and bred continuously in the Laboratory of Ecology of BASF AG in Ludwigshafen .
- Age at study initiation : 1 - 24 hours
- Feeding during test No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no

Test conditions

Test temperature:

20.3 - 21,5 °C

pH:

7,9 - 8,1

Dissolved oxygen:

8,4 - 8,6 mg O2/L

Nominal and measured concentrations:

Nominal concentrations:
0, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 ml)
- Fill volume: 10 ml
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
Illumination Artificial light, type warm white (e.g. OSRAM L58 W31) day:night rhythm = 16 : 8 h
- Light intensity: About 2 - 7 pE/(M2.s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Check of the study and recording (swimming ability) Visually after 0, 3, 6, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 12.5, 25, 50, 100 mg/L nominal

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50(24h) of the control substance potassium dichromate was 1,04 mg/I

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for the toxicity to Daphnia is greater than 100 mg/L

Executive summary:

The substance was tested to check its influence on the mobility to Daphnia Magna Straus according to EEC Directive 92/32/EEC (December 1992) equivalent or similar to OECD guideline 202. Four concentrations 12.5, 25, 50, 100 mg/L nominal were used.

After 48 hours no effect on the mobility of the Daphnia could be observed at each concentration. The EC50 is therefore greater than 100 mg/L.