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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
From July 31st to August 02nd, 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Justification for type of information:
Justification for Read Across is reported in the endpoint summary and it is further detailed in the report attached to the IUCLID section 13.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
part 1
GLP compliance:
not specified
Details on test solutions:
A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna, Stratus.
- Age at study initiation: young animals aged 0-2 4 hours were used.
- Food type: Tetra Animin®.
- Frequency of feeding: twice a week.

ACCLIMATION
- Acclimation conditions: daphnids were maintained at 20 ± 1°C and under a photoperiod of 16/8 hours light/dark. 5 organisms per litre.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 d
Test temperature:
20 ± 1 °C
pH:
Control: 7.9 - 8.6
Test vessels: 8.2 - 8.3
Dissolved oxygen:
Control: 7.8 - 8.8 mg/l
Test vessels: 7.8 - 8.4
Nominal and measured concentrations:
0 and 125 mg/l (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker according to DIN, HF.
- No. of organisms per vessel: 5.
- No. of vessels per concentration: 4 replicates.
- No. of vessels per control: 4 replicates.
- Biomass loading rate: use of 5 animals each in 20 ml of sample.

TEST MEDIUM
- Preparation of dilution water: M4 Medium.
- Composition of M4 Medium: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l, Na2SiO3 x 9H20 1000 µg/l, NaNO3 27.4 µg/l, KH2PO4 14.3 µg/l, K2HPO4 18.4 µg/l, Na2EDTA x 2H2O 2500 µg/l, FeSO4 x 7H2O 995.5 µg/l, H3BO3 2859.5 µg/l, MnCl2 x 4H2O 380.5 µg/l, LiCl 310.5 µg/l, RbCl 71.0 µg/l, SrCl2 x 6H2O 152.0 µg/l, NaBr 16.0 µg/l, Na2MoO4 x 2H2O 63.0 µg/l, CuCl2 x 6H2O 16.5 µg/l, ZnCl2 13.0 µg/l, CoCl2 x 6H2O 10.0 µg/l, KI 3.25 µg/l, Na2SeO3 2.19 µg/l, NH4VO3 0.575 µg/l, Thiamin x HCl 75.0 ng/l, Cyanocobalamin 1.0 ng/l, Biotin 0.75 ng/l
- Composition of dilution water: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l.
- Composition of tap water: Ca+ 31.6 mg/l, Mg2+ 5.5 mg/l, K+ 3.2 mg/l, Na+ 11.2 mg/l.

EFFECT PARAMETERS MEASURED
Immobilization after 48 hours.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Test results

Replicate Conc. (mg/l) N. of daphnids Inmmobilization after 24 hrs Inmmobilization after 48 hrs
Absolute Percentage Absolute Percentage
Blank 1 0 5 0 5 0 5
Blank 2 0 5 1 1
Blank 3 0 5 0 0
Blank 4 0 5 0 0
Test item 1 125 5 0 0 0 0
Test item 2 125 5 0 0
Test item 3 125 5 0 0
Test item 4 125 5 0 0

Summary of the test conditions

Conc. (mg/l) pH O2 (mg/l)
0 hrs 48 hrs 0 hrs 48 hrs
0 7.9 8.6 8.8 7.8
125 8.2 8.3 8.4 7.8
Validity criteria fulfilled:
yes
Remarks:
ion the control not more than 10 % of the Daphnia have been immobilized and the dissolved oxygen concentration at the end of the test was higher than 60 %
Conclusions:
EC50 (48h) > 125 mg/l (nominal)
Executive summary:

Method

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202, part I (Daphnia sp. Acute Immobilisation Test). A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.

Results

EC50 (48h) > 125 mg/l (nominal)

Description of key information

EC50 (48h) > 125 mg/l (nominal)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
125 mg/L

Additional information