1-chloro-3-nitrobenzene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity of mono-nitrobenzene derivatives in the Ames test and rec assay

Author:

Makoto Shimizu and Eiji Yan

Year:

1986

Bibliographic source:

Mutation Research, 170 (1986) 11-22

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

No significant lethal effect on the tester strain was observed in the presence or absence of S9 mix at any of the dose levels tested.

GLP compliance:

no

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-3-nitrobenzene

EC Number:

204-496-1

EC Name:

1-chloro-3-nitrobenzene

Cas Number:

121-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-3-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 1-Chloro-3-nitrobenzene
- Molecular weight (if other than submission substance): 157.56
- Smiles notation (if other than submission substance): c1(cc(ccc1)Cl)[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H4ClNO2/c7-5-2-1-3-6(4-5)8(9)10/h1-4H
- Substance type: Organic
- Physical state: Solid

Method

Target gene:

No data available

Species / strain

Species / strain / cell type:

S. typhimurium, other: Salmonella typhimurium TA98 and TA100.

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

The S9 fraction was prepared from the liver of PCB-treated male rats. The S9 mix (0.5 ml) contained 2 Micromoles NADPH, 2.5 micro moles G6P, 4 micro moles MgCl2, and 150 microliter S9 fraction containing 2.5 mg protein

Test concentrations with justification for top dose:

100microgram/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

DURATION
- Preincubation period:
20 min at 37 deg celcius

Evaluation criteria:

Dose response curves

Statistics:

No data available

Results and discussion

Test results

Species / strain:

S. typhimurium, other: S. typhimurium strain TA98 and TA100.

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data available

Remarks on result:

other: No mutagenic potential

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative Negative with and without

No significant lethal effect on the tester strain was observed in the presence or absence of S9 mix at any of the dose levels tested. No of His revertants in TA 98-(Without S9 mix) is 24,and in TA100 is 80. With activation-Without norharman-40, With norharman-69 In the presence of norharman, however, test chemical and exhibited mutagenicity to S.typhimurium TA98 in the presence of S9 mix indicating that norharman was effective only for the frame-shift mutagen.

Executive summary:

Study was conducted to test the effects of the given test chemical on salmonella on two strains TA 98 an TA100 using DMSO as a vehicle. Study is carried out based on Ames test with some modifications. Preincubation is done at 37 deg celcius for 20min.

No significant lethal effect on the tester strain TA 98 and TA 100 was observed in the presence or absence of S9 mix at any of the dose levels tested.