1-chloro-3-nitrobenzene

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Long term study was performed to test the effects of 1 Chloro-3-nitrobenzeneon early life stages of Zebra fish.Based on the teratogenic effects the EC50 was determined.

GLP compliance:

not specified

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- log Pow: 2.59

Analytical monitoring:

not specified

Details on sampling:

- Concentrations:10- 100mg/l

Vehicle:

yes

Details on test solutions:

- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Stock solutions of the compounds were prepared in dimethylsulfoxide DMSO

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish/Zebra Danio
- Age at study initiation (mean and range, SD):<4h
- Feeding during test: No feeding during test

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

7 d

Hardness:

250mg/l

Test temperature:

25 deg celcius

pH:

8.2

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

TEST SYSTEM
- Test vessel: 100ml glass dishes
- No. of organisms per vessel: 25 eggs per jar
- Renewal rate of test solution (frequency/flow rate): Alternate day
(Test solutions were refreshed every Monday, Wednesday and Friday).
-Photoperiod:12hr

Reference substance (positive control):

not specified

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

39 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Days to hatch and numbers hatched: Eggs hatch after 3-4 days
- Other biological observations Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as
deviations at the pectoral fins, abnormalities of the vertebrae and
size-increase of the yolk sac.

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

X2 test

Highest mortality was observed in egg-stage

Validity criteria fulfilled:

not specified

Conclusions:

Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as
deviations at the pectoral fins, abnormalities of the vertebrae and
size-increase of the yolk sac. Based on the effects the EC50 was determined to be 39mg/l. Classification may not be done as it is a chronic study.

Executive summary:

Freshly spawned eggs were obtained from a lab-culture. Within 4h after fertilization eggs were introduce to dishes containing test solution. Exposure duration was 7 days. Eggs were observed daily for abnormal changes.
Specific temperature ph and hardness were maintained and fishes were not feeded during the study period. The dose range used in 10-100mg/l, At given Effect concentration (39mg/l) some teratogenic and physiological abnormalities were observed predicting the toxicity of substance to fish early life stages.

Description of key information

Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as

deviations at the pectoral fins, abnormalities of the vertebrae and

size-increase of the yolk sac. Based on the effects the EC50 was determined to be 39mg/l. Classification may not be done as it is a chronic study.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Long term toxicity to fish:

Based on the value aquired from peer reviewed journal,long term toxicity of fish was discribed as below:

Freshly spawned eggs were obtained from a lab-culture. Within 4h after fertilization eggs were introduce to dishes containing test solution. Exposure duration was 7 days. Eggs were observed daily for abnormal changes.
Specific temperature ph and hardness were maintained and fishes were not feeded during the study period. The dose range used in 10-100mg/l, At given Effect concentration (39mg/l) some teratogenic and physiological abnormalities were observed predicting the toxicity of substance to fish early life stages.

Chronic study has been conducted for 7 days to test the effects of 1-chloro-3-nitrobenzene on danio rario.

Based on mortality observed for 7 days, the LC50 was determined to be 56 mg/l. Other than mortality some Delayed or inhibited embryonic development was also remarkable. Teratogenic effects on larvae were observed in concentrations at lethal-levels. These effects were noticed as deviations at the pectoral fins, abnormalities of the vertebrae and size-increase of the yolk sac.

Chronic study has been conducted for 14 days to test the effects of 1-chloro-3-nitrobenzene on danio rario.Based on mortality observed for 14 days, the LC50 was determined to be 15.4mg/l.

Chronic study has been conducted for 14 days to test the effects of 1-chloro-3-nitrobenzene onp reticullata.Based on mortality observed for 14 days, the LC50 was determined to be 20 mg/l & EC50 was determined to be 2.0 mg/l.

Based on the effect concentrations we can consider that the test substance 1-chloro-3-nitrobenzene has no chronic effect on fish and can be considered as not classified as per CLP.