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Toxicity to aquatic algae and cyanobacteria

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Description of key information

According to the results of the study compliant with the GLP and with the OECD TG 201, the  1,6-Divinylperfluorohexane is considered as not toxic for the aquatic organims at its limit of solubilty. therefore  1,6-Divinylperfluorohexane is not classified for the environmental part according to the Regulation (EC) 1272/2008.

Key value for chemical safety assessment

Additional information

One reliable key study is available for this endpoint (S.Hoger , 2010). In this study, the effect of the test item 1,6-Divinylperfluorohexane on the growth of the freshwater green algal species Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) was investigated in a 72‑hour static test according to OECD Guideline 201 (2006), and the method C.3. of Commission Regulation (EC) No 440/2008. The study was compliant with the GLP.

Following pre-experiments (non GLP), the test item 1,6-Divinylperfluorohexane was determined to be volatile and poorly soluble in the test medium, these pre-experiments were also performed to determine the optimum application method.

Therefore based on results of a pre-experiments and a preliminary range-finding test, a test was performed in closed system (i.e. the Erlenmeyer flasks were completely filled with test medium to keep the air-space in the test flasks as small as possible and modified and adapted test medium was used due to its volatility and the test solutions were prepared from a stock solution with a loading rate of 100 mg/L due to its poor solubility.

Pseudokirchneriella subcapitata was exposed to the following treatments: Dilution 1:100, 1:32, 1:10, 1:3.2 and the loading rate of 100 mg/L (three replicate flasks per concentration) and a control (six replicate flasks) for 72 hours, under constant illumination and shaking at a temperature of 22°C.


Samples of the algal populations were removed daily and Algal biomass determined for each control and treatment group, using afluorescence measurement (BIO-TEK Multi-Detection Microplate Reader, Model FLx800).


The measured concentrations of the test solutions at T0 hours were 0.52 and 1.9 mg/L for the dilution 1:3.2 and loading rate of 100 mg/L respectively and below the limit of quantification (LOQ) for the dilutions 1:100, 1:32 and 1:10. The measured concentrations of the test solutions at T= 72 hours showed a decrease of test item concentration in the test media since at the end of the test, the test item concentrations were below the LOQ in all samples.

No significant inhibitory effect on the growth of the algae (average growth rate and yield) during the test period of 72 hours at all the test concentration tested.


The 72-hour NOEC (growth rate and yield)was therefore determined to be at least the highest test concentration.The reported biological results were related to the mean measured test item concentrations calculated as the geometric means of the concentrations measured at the start and the end of the test As the concentrations at the end of the test were below the LOQ of 0.134 mg test item/L, the geometric mean was calculated from the concentrations at the start of the test and half the LOQ at the end of the test.


The results obtained with loading rates were as follows:

- 72h-ErL50 ≥ 100 mg/L (growth rate)

- 72h-EbL50 ≥ 100 mg/L (yield)

- 72h-NOELR ≥ 100 mg/L (growth rate and yield)


The results obtained with the geometric mean of the measured concentrations were as follows:

- 72h-ErC50 ≥ 0.35 mg/L (growth rate)

- 72h-EbC50 ≥ 0.35 mg/L (yield)

- 72h-NOEC ≥ 0.35 mg/L (growth rate and yield)


It could be concluded that no toxicity was observed at the limit of solubility of the test item 1,6-Divinylperfluorohexane in the test medium.


Finally, according to the Guidance on the Application of the CLP Criteria, as the toxicity of the 1,6-Divinylperfluorohexane is above the limit of the solubility in the test medium and as the best technic was used to achieve the maximum dissolved concentrations, 1,6-Divinylperfluorohexane should not be classified in accordance with the Regulation (EC) No 1272/2008