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Short-term toxicity to aquatic invertebrates

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Description of key information

According to the results of the study compliant with the GLP and with the OECD TG 202, the  1,6-Divinylperfluorohexane is considered as not toxic for the aquatic organims at its limit of solubilty. Therefore  1,6-Divinylperfluorohexane is not classified for the environmental part according to the Regulation (EC) 1272/2008.

Key value for chemical safety assessment

Additional information

One reliable key study is available for 1,6-Divinylperfluorohexane for this endpoint. In this acute toxicity study (S. Hoger, 2010), the acute immobilization (EC50 and NOEC) of the test item 1,6-Divinylperfluorohexane to daphnia magna (STRAUS) was determined according to the method C.2 of the Commission Regulation (EC) No 440/2008 and the OECD Guideline 202. The study was compliant with the GLP.The study was conducted under semi-static conditions over a duration of 48 hours.


Following pre-experiments (non GLP), the test item 1,6-Divinylperfluorohexane was determined to be volatile and poorly soluble in the test medium, these pre-experiments were also performed to determine the optimum application method.

Therefore, based on results of a pre-experiments and a preliminary range-finding test, a limit test was performed in closed system (i.e. glass tubes completely filled with test medium) due to its volatility and the limit test solution was prepared from a solution with a loading rate of 100 mg/L due to its poor solubility.

20 test organisms, divided into four replicates of 5 daphnids each, were exposed to the limit test concentration (loading rate of 100 mg/L) and control and were observed for immobility after 24 and 48 hours of exposure. The test solutions were renewed after 24 hours.


The measured concentrations of the limit test solution (loading rate of 100 mg/L) were 1.1 and 0.48 mg/L at 0 and 24 hours (i.e. start of the renewal periods) and 0.2 and 0.21 mg/L at 24 and 48 hours (end of the renewal periods) respectively.

No immobilized test organisms were observed during the test period of 48 hours in the control and in the limit test solution.

The 48-hour NOEC was therefore determined to be the limit test concentration.The reported biological results were related to the loading rate of 100 mg/L and to the arithmetic mean of the geometric means of both 24h-periods (0.40 mg/L).


The results obtained with loading rates were as follows:

- 48h-EL50 ≥ 100 mg/L

- 48h-NOELR ≥ 100 mg/L


The results obtained with the geometric mean of the measured concentrations were as follows:

- 48h-EC50 ≥ 0.40 mg/L

- 48h-NOEC ≥ 0.40 mg/L


It could be concluded that no toxicity was observed at the limit of solubility of the test item 1,6-Divinylperfluorohexane in the test medium.


Finally, according to the Guidance on the Application of the CLP Criteria, as the toxicity of the 1,6-Divinylperfluorohexane is above the limit of the solubility in the test medium and as the best technic was used to achieve the maximum dissolved concentrations, 1,6-Divinylperfluorohexane should not be classified in accordance with the Regulation (EC) No 1272/2008.