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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

in vitro gene mutation study in bacteria
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Peer reviewed data, acceptable for assessment.

Data source

Reference Type:
secondary source
Genotoxic activity and potency of 135 compounds in the Ames reversion test and in a bacterial DNA-repair test.
De Flora S. et al.
Bibliographic source:
OECD SIDS-Draft; original source: Mutat. Res., 133, 161-198

Materials and methods

Principles of method if other than guideline:
no data
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
nickel nitrate
nickel nitrate
Details on test material:
- Name of test material (as cited in study report): nickel nitrate


Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA1535, TA100, TA1538, TA98, TA1537, TA97
Metabolic activation:
with and without
Test concentrations with justification for top dose:
no data
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA1535, TA100, TA1538, TA98, TA1537, TA97
Metabolic activation:
with and without
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Migrated from field 'Test system'.

Any other information on results incl. tables

Negative results were seen in the bacterial reverse mutation assay.

Applicant's summary and conclusion