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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented and acceptable publication

Data source

Reference
Reference Type:
publication
Title:
pH-DEPENDENT TOXICITY OF Cd, Cu, Ni, Pb AND Zn 10 CERIODAPHNIA DUBIA, PIMEPHALES PROMELAS, HYALELLA AZTECA AND LUMBRJCULUS VARIEGATUS
Author:
Schubauer-Berigan et al.
Year:
1993
Bibliographic source:
Envrionmental Toxicology and Chemistry. Vol. 12, Ptp. 1261-1266

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Nickel dichloride
EC Number:
231-743-0
EC Name:
Nickel dichloride
Cas Number:
7718-54-9
IUPAC Name:
nickel(2+) dichloride
Details on test material:
- Name of test material (as cited in study report): Nickelchloride

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: Test organisms were cultured at the U.S. EPA laboratorys in Duluth, Minnesota
- Age at study initiation (mean and range, SD): < 48 h
- Feeding during test
- Food type: 100 µg of a yeast-Cerophyl (Agri-Tech, Kansas City,MO)-fermented Purina Brand (St. .Louis, MO)



Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
300 mg/L Ca CO3
Test temperature:
25 °C
pH:
three pH values:
6.3, 7.3, 8.3
Dissolved oxygen:
never below 5 mg/L (60 % saturation)
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2-3
- No. of vessels per control (replicates): 2-3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted hard water
- Alkalinity: 225-245 mg/L CaCo3



OTHER TEST CONDITIONS
- Photoperiod: 16:8 light:dark cycle
- Adjustment of pH: yes, with HCL


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 0.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Nickel
Basis for effect:
mortality
Remarks on result:
other: pH: 6.0-6.5
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.14 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Nickel
Basis for effect:
mortality
Remarks on result:
other: pH: 7.0-7.5
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.013 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Nickel
Basis for effect:
mortality
Remarks on result:
other: pH: 8.0-8.5
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 0.3 mg/L
Conc. based on:
other: Nickeldihydroxide
Basis for effect:
mortality
Remarks on result:
other: pH: 6.0-6.5. Result calculated for Nickeldihydroxide
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.22 mg/L
Conc. based on:
other: Nickeldihydroxide
Basis for effect:
mortality
Remarks on result:
other: pH: 7.0-7.5. Result calculated for Nickeldihydroxide
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.02 mg/L
Conc. based on:
other: Nickeldihydroxide
Basis for effect:
mortality
Remarks on result:
other: pH: 8.0-8.5. Result calculated for Nickeldihydroxide

Applicant's summary and conclusion