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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Toxicity to fish:


Short-term toxicity to fish:

The acute toxicity of trimethylolpropane to the marine bleak (Alburnus alburnus) was determined in a static test with brackish water (7‰ salinity, pH 7.8) and with an exposure duration of 96 h. Six different concentrations, not further specified, were tested.Analytical monitoring was not performed. The 96-h LC50 was determined to be greater than 1000 mg/L as reported by Bengtsson and > 10,000 mg/L as reported by Linden (based on nominal concentrations).

The study satisfies the guideline requirements for an acute toxicity study with fish.

Reference: Bengtsson & Tarkpea, 1983, Linden et al., 1979


One of the supporting studies was performed with the freshwater species Oryzias latipes and resulted in the same endpoint: 96-h LC50 > 1000 mg/L. Reference: EA Japan /SIDS, <=1994 (Reliability 2).

Since the publications by Bengtsson & Tarkpea and Linden et al. are more detailed than the data in the SIDS on the study from EA Japan, they were chosen as key study.

Long-term toxicity to fish:

Due to the very low toxicity of trimethylolpropane to fish and a low potential to bioaccumulate in fish, a long-term toxicity test on fish is not needed.

Toxicity to aquatic invertebrates:

Short-term toxicity to aquatic invertebrates:

The 48-hr-acute toxicity of trimethylolpropane to Daphnia magna was studied under static conditions. Daphnids were exposed to the substance at nominal concentrations of 5,000; 7,500; 10,000; 15,000, 20,000, 25,000; 40,0000 mg/L. Immobilization was observed at 24 and 48h. The 48-hour EC50 was 13,000 mg/L (based on nominal concentrations). This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

Reference: Walton, J.R. and, E.M., 1980


Long-term toxicity to aquatic invertebrates:

A static 21-d test was performed with trimethylolpropane on Daphnia magna by following an OECD Guideline.

A NOEC > 1000 mg/L was determined.

Being performed by EA Japan according to a OECD-Guideline, the study is considered to be acceptable.

Reference: EA Japan / SIDS, <=1994


Toxicity to algae:


The toxicity of trimethylopropane on the green algae Pseudokirchneriella subcapitata (reported as Selenastrum carpicornutum) was tested according to an OECD guideline. The 72-h EbC50 was determined to be greater than 1000 mg/L (based on nominal concentrations).

Being performed by EA Japan according to a OECD-Guideline, the study is considered to be acceptable.

Reference: EA Japan/SIDS, <=1994


Toxicity to microorganisms (activated sludge):


The respiration inhibition of activated sludge from predominantly domestic sewage treatment plant by trimethylolpropane was determined according to the EU Guideline C.11 with an incubation time of 3 hours. The concentrations tested were 10, 100 and 1000 mg/L. EC50 of the reference substance 3,5 - dichlorophenol was 9.2 mg/L ( 95% C.I. 3 - 24 mg/L). The respiration rates of the 2 controls differed less than 15% from each other.

The EC50 and the EC10 for trimethylolpropane were determined to be > 1000 mg/L.

The validity criteria of the guideline are fulfilled.

Reference: Neuhahn, 2010


Toxicity to other aquatic organisms (fish larvae):

The acute toxicity was tested in a static test on larvae of Petromyzon marinus. A 24h-LC0 was determined to be > 5 mg/L.

The study does not meet important criteria of today's standards and is therefore notc onsidered to be reliable.

Reference: Applegate, 1957