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EC number: 266-028-2 | CAS number: 65996-93-2 The residue from the distillation of high temperature coal tar. A black solid with an approximate softening point from 30°C to 180°C (86°F to 356°F). Composed primarily of a complex mixture of three or more membered condensed ring aromatic hydrocarbons.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: long-term
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Soil ecotoxicity of polycyclic aromatic hydrocarbons in relation to soil sorption, lipophilicity, and water solubility
- Author:
- Sverdrup L E, Nielsen T, Krogh P H
- Year:
- 2 002
- Bibliographic source:
- Environ. Sci. Technol. 2002, 36, 2429-2435 (2002)
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
- GLP compliance:
- no
- Application method:
- soil
Test material
- Reference substance name:
- Benzo[def]chrysene
- EC Number:
- 200-028-5
- EC Name:
- Benzo[def]chrysene
- Cas Number:
- 50-32-8
- Molecular formula:
- C20H12
- IUPAC Name:
- benzo[pqr]tetraphene
- Reference substance name:
- benzo(a)pyrene
- IUPAC Name:
- benzo(a)pyrene
- Details on test material:
- Name of test material: benzo(a)pyrene
- Source: Sigma-Aldrich, St. Louis, MO
- Purity: >98 %
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 9.4*10^-4 Pa (at 25 °C)
- Water solubility: 50 - 70 µg/L (20 - 25 °C)
- Henry's law constant: 4.3 Pa*m3/mol (at 25 °C)
- log Pow: 4.3 - 4.5
- pKa: not applicable
- Stability in water: limited due to photolytic decomposition and evaporation
- Stability in light: instable in the presence of UV
- pH dependance on stability: not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Soil samples of 10, 100, and 1000 mg/kg soil (dw)
Analysis at start and at the end of experiment
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Acetone (4ml) with test substance was added to each container (26g dw soil/ container)
Vehicle control samples: acetone (4ml) without test substance was applied
Evaporation of acetone (fume hood, 24h) before use
Test organisms
- Test organisms (species):
- other: Folsomia fimetaria
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- TEST ORGANISM
- Source: synchronized animals, no further information
- Age at test initiation (mean and range, SD): 23-26 d
- Weight at test initiation (mean and range, SD): no data
Study design
- Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 20 ±1 °C
- pH (if soil or dung study):
- see details below
- Humidity:
- 50 % of water holding capacity
- Photoperiod and lighting:
- - Photoperiod: 12:12 h (light:dark)
- Light intensity: 400-800 lux - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): no data
- Amount of soil or substrate: 26g +/- 0.05g artificial soil
- No. of organisms per container (treatment): 10 males, 10 females
- No. of replicates per treatment group: 4
- No. of replicates per vehicle control: 4
Artificial soil test substrate: Danish agricultural soil (Askov, Jutland)
- Composition: Sandy loam
Coarse sand (0.2 – 2 mm) 38.4 %, fine sand (0.063 – 0.2 mm) 23.6 %, coarse silt (0.02 – 0.063 mm) 10.0 %, fine silt (0.002 – 0.02 mm) 12.3 %,
clay (<0.002 mm) 13 %, humus 2.8 %,
Organic carbon content: 1.6 %,
Soil pH 6.2, no adjustment
Density 1.135 g/cm3,
Cation exchange capacity 8.14 meq/100 g
Samples were sieved through a 2 mm mesh before use
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h (light/dark)
- Light intensity: 400-800 lux
EFFECT PARAMETERS MEASURED: Mortality, reproduction at end of exposure time (21 d)
VEHICLE CONTROL PERFORMED: yes (details see above) - Nominal and measured concentrations:
- Nominal: 3, 10, 30, 100, 300, and 1000 mg/kg soil (dw)
Measured for 10, 100, and 1000 mg/kg soil (dw), recovery: 8.3, 79, and 840 mg/kg soil (dw) prior to exposure
Ratio measured/ nominal concentrations: 81.7 %
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 840 mg/kg soil dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 840 mg/kg soil dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Measured concentrations of test substance after test period (21 d):
10 mg/ kg soil (dw) nominal: 5.8 mg/ kg soil (dw)
100 mg/ kg soil (dw) nominal: 66 mg/ kg soil (dw)
1000 mg/ kg soil (dw) nominal: 840 mg/ kg soil (dw)
Ratio measured/ nominal concentrations: 69.2 % - Reported statistics and error estimates:
- EC10 values (reproduction): estimated by logistic regression by SAS/STAT PROCLIN
LC50 values (mortality): estimated using probit analysis in SAS/STAT
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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