Pitch, coal tar, high-temp.

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: AFNOR (draft, not further specified)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Benz[a]anthracene

EC Number:

200-280-6

EC Name:

Benz[a]anthracene

Cas Number:

56-55-3

Molecular formula:

C18H12

IUPAC Name:

tetraphene

Constituent 2

Reference substance name:

Benzo[def]chrysene

EC Number:

200-028-5

EC Name:

Benzo[def]chrysene

Cas Number:

50-32-8

Molecular formula:

C20H12

IUPAC Name:

benzo[pqr]tetraphene

Constituent 3

Reference substance name:

benzo[a]pyrene / benzo[def]chrysene

IUPAC Name:

benzo[a]pyrene / benzo[def]chrysene

Details on test material:

- Name of test material (as cited in study report): benzo(a)pyrene
- Molecular formula (if other than submission substance): C20H12
- Molecular weight (if other than submission substance): 252
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in coal-tar pitch: 1.0 - 1.3 % (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier )

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 7.3*10^-7 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 1.5 - 3.8 µg/L (20 - 25 °C);
soluble portion of the WSF of coal-tar pitch (loading 100 mg/L): [see 4.8: NOACK 2009_WSF, 100 mg/L nominal]
- Henry's law constant: 0.034 Pa*m3/mol (at 25 °C)
- log Pow: = 6.13 - 6.5
- pKa: not applicable
- Stability in water: limited due to photolytic decomposition and evaporation
- Stability in light: instable in the presence of UV
- pH dependance on stability: not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

no data

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Test conditions

Hardness:

240 mg/L (as CaCO3)

Test temperature:

25 +- 2°C

pH:

8.1 +-0.4

Dissolved oxygen:

>7.0 mg/L

Nominal and measured concentrations:

Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium,
based on an arithmetic model (OECD 1999) (see Report, p. 44/45).

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 +-100 µs/cm


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: no data

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

EC10

Effect conc.:

0.503 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

dissolved

Remarks:

test material

Basis for effect:

other: mortality and reproduction

Effect concentrations 2

Duration:

7 d

Dose descriptor:

EC10

Effect conc.:

0.77 µg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Remarks:

test material

Basis for effect:

other: mortality and reproduction

Remarks on result:

other: 95%-C.I.: 0.03 - 2.2 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes