Registration Dossier

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: AFNOR (draft, not further specified)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benz[a]anthracene
EC Number:
200-280-6
EC Name:
Benz[a]anthracene
Cas Number:
56-55-3
Molecular formula:
C18H12
IUPAC Name:
tetraphene
Constituent 2
Chemical structure
Reference substance name:
Benzo[def]chrysene
EC Number:
200-028-5
EC Name:
Benzo[def]chrysene
Cas Number:
50-32-8
Molecular formula:
C20H12
IUPAC Name:
benzo[pqr]tetraphene
Constituent 3
Reference substance name:
benzo[a]pyrene / benzo[def]chrysene
IUPAC Name:
benzo[a]pyrene / benzo[def]chrysene
Details on test material:
- Name of test material (as cited in study report): benzo(a)pyrene
- Molecular formula (if other than submission substance): C20H12
- Molecular weight (if other than submission substance): 252
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in coal-tar pitch: 1.0 - 1.3 % (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier )
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 7.3*10^-7 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 1.5 - 3.8 µg/L (20 - 25 °C);
soluble portion of the WSF of coal-tar pitch (loading 100 mg/L): [see 4.8: NOACK 2009_WSF, 100 mg/L nominal]
- Henry's law constant: 0.034 Pa*m3/mol (at 25 °C)
- log Pow: = 6.13 - 6.5
- pKa: not applicable
- Stability in water: limited due to photolytic decomposition and evaporation
- Stability in light: instable in the presence of UV
- pH dependance on stability: not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Hardness:
240 mg/L (as CaCO3)
Test temperature:
25 +- 2°C
pH:
8.1 +-0.4
Dissolved oxygen:
>7.0 mg/L
Nominal and measured concentrations:
Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium,
based on an arithmetic model (OECD 1999) (see Report, p. 44/45).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 +-100 µs/cm


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
0.503 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
0.77 µg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: 95%-C.I.: 0.03 - 2.2 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes