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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was given a reliability rating of 1 because it followed a standard test guideline, which describes a procedure to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
Individual treatment solutions were prepared as Water Accommodated Fractions (WAFs). This included adding test substance to glass vessels,
which contained dilution water and mixing for approximately 24 hours with a magnetic stirrer to produce a series of geometrically-spaced
loading rates (mg/L). After mixing, the WAFs were allowed to settle for an hour. Each aqueous phase was then drawn off and transferred to two
150 mL Erlenmeyer flasks which were completely filled. Two further flasks were filled with dilution water only and served as controls.
Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms were supplied by in-house cultures. The laboratory culture is derived from a clone held at ICI Brixham Laboratory. The ICI clone was
itself obtained from the Institut de Recherche Chimique Applique (I.R.Ch.A.), France. Test organisms age = <24 hours old; parents age = 15 - 28
days.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
Water hardness of the control dilution water was 174 mg/L as CaCO3.
Test temperature:
Test temperature was 20 – 22 ºC.
pH:
The pH ranged from 7.7 to 8.1.
Dissolved oxygen:
Dissolved oxygen was 8.3 to 8.6 mg/L for "new" solutions and 8.6 to 8.7 mg/L for "old" solutions.
Salinity:
freshwater
Nominal and measured concentrations:
Nominal treatment levels were control, 0.1, 0.4, 1.0, 3.0 and 10 mg/L, which at t=0 measured non detected (< 2.5 mg/L), NA (not analyzed), NA, NA,
3.3 mg/L and 8.2 mg/L, respectively.
Details on test conditions:
Ten Daphnia magna, less than 24 h old, were added to each flask. The flasks were sealed with black caps that excluded air from the vessel. After
24 and 48 h the numbers of immobilized D.magna were recorded. D.magna were considered to be immobile, if, when the contents of the flask were
swirled they did not swim during a 15 second period of observation.

Photoperiod with a 16 h : 8 h light : dark lighting regime.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
>= 3 - <= 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
See Table in Remarks on Results
Reported statistics and error estimates:
Loading rates causing a 50% effect were determined by visual inspection of the data. They are expressed as being within a range bounded by loading rates that were tested. This is considered more appropriate for test materials that are mixtures of substances of different solubilities than is an interpolative statistical technique.

Nominal Loading Measured Conc  Daphnia Immobilized
(mg/L)  (mg/L)   (@ 24, 48 hrs)*
     
Control  <LoD 0,0
0.1 <LoD  0, 0
0.4 <LoD  0, 0
1.0 <LoD  0, 0
3.0  3.3 – 3.1 ** 0, 0
10.0  8.2 – 7.8 **  10, 10

*10 daphnids added to each of the control and treatment levels (both in duplicate) at test initiation

** Measured concentrations refer to the t=0h (fresh) and t=48 h (old) samples

Validity criteria fulfilled:
yes
Conclusions:
Water accommodated fractions of hydrocarbons, C10, aromatics, >1% naphthalene, produced a 48-hour LL50 value between 3 and 10 mg/L with Daphnia magna.
Executive summary:

Water accommodated fractions of hydrocarbons, C10, aromatics, >1% naphthalene, produced a 48-hour LL50 value between 3 and 10 mg/L with Daphnia magna.

Description of key information

Water accommodated fractions of hydrocarbons, C10, aromatics, >1% naphthalene, produced a 48-hour LL50 value between 3 and 10 mg/L with Daphnia magna.

 

In a supporting study, water accommodated fractions of hydrocarbons, C10, aromatics, >1% naphthalene, produced a 48-hour LL50 value of 10 mg/L with Daphnia magna.

Key value for chemical safety assessment

Additional information

Water accommodated fractions of hydrocarbons, C10, aromatics, >1% naphthalene, produced a 48-hour LL50 value between 3 and 10 mg/L with Daphnia magna.

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