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EC number: 700-195-9 | CAS number: 223398-24-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- [TN]Gro 266[/TN][SPEC][/SPEC][AM][/AM]
- IUPAC Name:
- [TN]Gro 266[/TN][SPEC][/SPEC][AM][/AM]
- Reference substance name:
- 4-acetyl-1-methylpyridin-1-ium 4-methylbenzene-1-sulfonate
- EC Number:
- 700-195-9
- Cas Number:
- 223398-24-1
- Molecular formula:
- C8 H10 N O . C7 H7 O3 S
- IUPAC Name:
- 4-acetyl-1-methylpyridin-1-ium 4-methylbenzene-1-sulfonate
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- Dose levels are in terms of the test item as supplied by the sponsor.
The dose formulations were made shortly before each dosing occasion using a magnetic stirrer and a spatula as homogenizers.
The test item was weighed into a tared glass beaker on a suitable precision balance and the vehicle added (weighed: volume).
Homogeneity of the test item in the vehicle was maintained during administration using a magnetic stirrer. - Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- not specified
- Statistics:
- No statistical analysis was used.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- No deaths occured during the study.
- Clinical signs:
- other: One hour after treatment all female animals showed slightly ruffled fur which persisted up to the 5-hour reading. In the male animals slightly ruffled fur was observed 5 hours after treatment. There were no furthere clinical signs noted until test day 15,
- Gross pathology:
- No macrosopic findings were recorded at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified EU DSD and UN GHS
- Remarks:
- Criteria used for interpretation of results: EU
- Conclusions:
- The median lethal dose of GRO 266 after single oral administratin to rats of both sexes, observed over a period of 14 days is:
LD50(rat): greather than 2000 mg/kg body weight
This result corresponds to Category 5 or Unclassified based on the GHS classification criteria and an LD50 cut off of 5000 mg/kg body weight according to the OECD 423 Test Procedure. - Executive summary:
Two groups, one of three female and one of three male HanRcc: WIST(SPF) rats, were treated with GRO 266 by oral gavage administration at a dosage of 2000 mg/kg bw. The test item was diluted in vehicle (purified water) at a concentration of 0.2 g/ml and administered at avolume of 10 ml/kg.
The animals were examined daily during the acclimatization period and mortality, viability and clinical signs were recorded. All animals were examined for clinical signs within the first 30 minutes and approximately 1,2,3 and 5 hours after treatment on day 1 and ance daily during test days 2 -15. Mortality/viability was recorded at approximately 30 minutes, 1,2,3 and 5 hours after administration on test day 1 (with the clinical signs) and twice daily during days 2 -15. Body weights were on day 1 (prior to administration) and on days 8 and 15. All animals were necropsied and examined macroscopally.
All animals survived until the end of the study period.
One hour after treatment all female animals showed slightly ruffled fur which persisted up to the 5-hour reading. In the male animals slightly ruffled fur was observed 5 hour after treatment. There were no further clinical signs noted until test day 15, the end of observation time.
The body weight of the animals was within the range commonly recorded for this strain and age.
No macroscopic findings were recorded at necropsy.
The median lethal dose of GRO 266 after single oral administration to rats of both sexes, observed over a period of 14 days is:
LD50(rat): greather than 2000 mg/kg body weight
This result corresponds to Category 5 or Unclassified based on the GHS classification criteria and an LD50 cut off of 5000 mg/kg body weight according to the OECD 423 Test Procedure.
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