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EC number: 700-195-9
CAS number: 223398-24-1
Assessment for Contact Hypersensitivity to
GRO 266 in the Mouse (Local Lymph Node Assay),
The study was carried out based on the
guidelines described in:
OECD, Section 4, Health Effects, No. 429
EC, Council Directive 67/548/EEC, Annex V,
EPA, OPPTS 870.2600 (2003) "Skin
Test Substance concentrations selected for
the main study were based on the results of a preliminary study.
In the main study, three groups of five
experimental animals were treated with test substance concentration of
10%, 25% and 50% on three consecutive days, by open application on the
ears. Five vehicle control animals were similarly treated, but with
vehicle alone (Ethanol/water). Three days after the last exposure, all
animals were injected with 3H-methyl thymidine and after five hours the
draining (auricular) lymph node cells excised.
After precipitating the DNA of the lymph
node cells, radioactivity measurements were performed. The activity was
expressed as the number of Disintegrations Per inute (DPM) and a
stimulation index (SI) was subsequently calculated for each group.
Slight irritation was observed in one ear of
one animal at 50%. No oedema was observed in any of the animals
examined. The irritation of the ears as shown by one animal was
considered not to have a toxicologically significant effect on the
activity of the nodes.
The majority of nodes were considered normal
in size, expect for the nodes of one animal treated at 10 %, two animals
treated at 25 % and one animal at 50 %. No macrosopic abnormalities of
the aurrounding area were noted.
Mean DPM/animal values for the experimenta
groups treated with substance concentrations 10, 25 and 50% were 142,
334 and 181 respectively. The mean DPM/animal value for the vehicle
control group was 143.
The SI values calculated for the substance
concentrations 10, 25 and 50 % were 1.0, 2.3 and 1.3 respectively.
Since there was no indicated thet the test
substance elicit an SI>3 when tested up to 50%, GRO 266 was considered
to be a non skin sensitizer.
No ECETOC classification is applicable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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