Allyl 2,3-epoxypropyl ether

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Biotransformation of the double bond in allyl glycidyl ether to an epoxide ring. Evidence from hemoglobin adducts in mice.

Author:

Pérez HL and Osterman-Golkar S

Year:

2000

Bibliographic source:

Chemico-biological interactions 125: 17-28. Cited in OECD SIDS (SIAM 25, October 2007, Helsinki)

Materials and methods

Principles of method if other than guideline:

Eleven male mice (C3H/Hej) were administered 4 mg/mouse of the test substance dissolved in acetone, by skin application. Adducts to N-terminal valine in hemoglobin were analyzed using a modified Edman method for derivatization and using gas chromatography/tandem mass spectrometry for detection.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Allyl 2,3-epoxypropyl ether

EC Number:

203-442-4

EC Name:

Allyl 2,3-epoxypropyl ether

Cas Number:

106-92-3

Molecular formula:

C6H10O2

IUPAC Name:

2-[(prop-2-en-1-yloxy)methyl]oxirane

Details on test material:

- Name of test material (as cited in study report): Allyl glycidyl ether (AGE)
- Analytical purity: 99%

Radiolabelling:

no

Test animals

Species:

mouse

Strain:

C3H

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Deutschland GmbH, Sulzfeld, Germany or bred at the Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden
- Average weight at study initiation: 28 g
- Diet (e.g. ad libitum): standard pellet diet
- Water (e.g. ad libitum): tap water

Administration / exposure

Type of coverage:

open

Vehicle:

acetone

Duration of exposure:

5 or 24 h

Doses:

4 mg/mouse

No. of animals per group:

6

Control animals:

yes

Results and discussion

Any other information on results incl. tables

Adducts of diglycidyl ether (I) or 2,3-dihydroxypropyl glycidyl ether (III) with N-terminal valine, N-(2-hydroxy-3-(2,3-dihydroxy)propyloxy) propylvaline (diOHPrGEVal) was below the detection limit of the analytical method, 20 pmol/g globin (n=5). The level of AGEVal (AGE reacted with N-terminal valine) in mice administered AGE by skin application (n=6) was about 20 pmol/g globin. Neither AGEVal nor diOHPrGEVal were detected in control animals.

Table: Hemoglobin adducts formed from AGE and its metabolites 2,3-dihydroxypropyl glycidyl ether or diglycidyl ether in mice treated with AGE.

Supplier of mice	Time after treatment (h)	AGEVal (pmol/g globin)	diOHPrGEVal (pmol/g globin) Mean (±)
Charles River	5	18; 27 (a)	n.d (b)
Charles River	24	23; 99 (a)	n.d
Stockholm	24	9; 15 (a)	n.d
Stockholm and   Charles River	24	n.d	<20 (n =5) (c)
Charles River	5*	n.d	<20 (n =3)
Stockholm and   Charles River	5*	<2 (n =6)	n.d

(a) data for individual animals; (b) not determined; (c) n denotes the number of mice; * control groups