Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study. Biodegradation study, adverse effects on microorganisms derived from toxicity control

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 301 D including toxicity control
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Allyl glycidyl ether
- Physical state: clear colourless liquid
- Analytical purity: 99.58%
- Lot/batch No.: S22491
- Expiration date of the lot/batch: 14 August 2007
- Stability under test conditions: not indicated
- Storage condition of test material: at room temperature in the dark

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands
- Method of cultivation: Secondary effluent was fitlered through a coarse filter paper, the first 200 mL was discarded. The filtrate was kept aerated until inoculation. 4 mL filtrate of secondary effluent per litre of final volume

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d

Test conditions

Details on test conditions:
TEST CONDITIONS
- Test temperature: between 21.9 and 22.8 °C
- pH: at test start between 7.4 and 7.5
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 250 - 300 mL BOD bottles with glass stoppers
- Number of culture flasks: 2
- Measuring equipment: WTW inolab Oxi Level 2 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G
- Test performed in open system: no

SAMPLING
- Sampling frequency: samples taken at day 0, 7, 14, 21 and 28
- Sampling method: Direct measurement of oxygen content

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
EC0
Effect conc.:
1.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

Biodegradation

Sample

Concentration

% Biodegradation after

 

 

0

7

14

21

28

Reference substance

2.0 mg/L

0

47

76

71

77

Test substance

1.5 mg/L

0

-11

0

6

9

Toxicity control (Reference substance + test substance)

2.0 mg/L + 1.5 mg/L

0

13

27

23

29

Accroding to OECD guideline 301, in a toxicity control at least 25% degradation have to be observed after 14 days of exposure. If this criterion is fulfilled the test substance is considered to be not inhibitory to activated sludge.

Applicant's summary and conclusion