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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given, acceptable with restrictions (The study was performed before the establishment of GLP and OECD guidelines.)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
The toxicology of Glycidol and some Glycidyl Ethers.
Author:
Hine CH et al.
Year:
1956
Bibliographic source:
Report, Department of Pharmacology and Experimental Therapeutics, University of California School of Medicine, San Francisco. Cited in OECD SIDS (SIAM 25, October 2007, Helsinki)
Reference Type:
publication
Title:
The toxicology of Glycidol and some Glycidyl Ethers.
Author:
Hine CH, Kodama JK, Wellington JS, Dunlap MK and Anderson HH
Year:
1956
Bibliographic source:
A.M.A. Archives of Industrial Health. 14: 250-264. Cited in OECD SIDS (SIAM 25, October 2007, Helsinki)
Reference Type:
publication
Title:
The toxicology of glycol and some glycidyl ethers.
Author:
Shell Chemical Company
Year:
1956
Bibliographic source:
TSCATS, OTS0523687, New Doc ID 40-5640496, Date produced 3/18/56.

Materials and methods

Principles of method if other than guideline:
According to the method of Draize JH (Food Drug & Cosmetics Law J. 10: 722-732, 1955)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Allyl Glycidyl Ether

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Simonson laboratory in Gilroy, California
- Weight at study initiation: 2-3 kg
- Diet (e.g. ad libitum): standards rabbit pellets

Test system

Type of coverage:
occlusive
Preparation of test site:
other: clipped
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml
Duration of treatment / exposure:
24 hour(s)
Observation period:
72 h
Number of animals:
3
Details on study design:
TEST SITE
- Area of exposure: not reported
- The back and flanks of rabbits were clipped 24 h prior to use. The shoulder and hips were used as test sites, two areas on each animal being scarified and two intact. Scarification was accomplished by making four intersecting epidermal scratches, each about 2 cm long, with a scalpel blade. A patch consisting of three layers of gauze was secured over each area with adhesive tape, and undiluted compound was introduced under the gauze. The rabbits were wrapped in towels and immobilized for 24 h, after which the areas were examined and irritation scores noted.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no

SCORING SYSTEM: according to Draize JH (1955)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Remarks:
Intact skin
Basis:
mean
Time point:
other: 24-72 h
Score:
1.33
Max. score:
4
Reversibility:
no data
Remarks on result:
other: see free text for individual scores
Irritation parameter:
edema score
Remarks:
Intact skin
Basis:
mean
Time point:
other: 24-72 h
Score:
1.83
Max. score:
4
Reversibility:
no data
Remarks on result:
other: see free text for individual scores

Any other information on results incl. tables

Irritation scores

Animal No.

Time period

(h)

Scarified skin

Intact skin

Erythema

Edema

Erythema

Edema

1

24

2

1

1

4

72

3

2

2

2

2

24

4

1

3

0

72

4

4

0

0

3

24

1

3

1

3

72

2

4

1

2

1

Mean (24-72 h)

2.5

1.5

1.5

3.0

2

Mean (24-72 h)

4.0

2.5

1.5

0.0

3

Mean (24-72 h)

1.5

3.5

1.0

2.5

1-3

Total mean

2.67

2.50

1.33

1.83

Applicant's summary and conclusion