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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1966
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment (no information on test item purity and composition, no information on exposure duration, no details on animals and housing conditions), deviating from existing guidelines (only 3 animals/group)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966

Materials and methods

Principles of method if other than guideline:
test for acute dermal toxicity
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
EC Number:
213-879-2
EC Name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
Cas Number:
1047-16-1
Molecular formula:
C20H12N2O2
IUPAC Name:
5,12-dihydroquino[2,3-b]acridine-7,14-dione
Test material form:
not specified

Test animals

Species:
rabbit
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
not specified
Vehicle:
not specified
Duration of exposure:
no data
Doses:
2 mL/kg bw
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
The test substance was applied onto intact or abraded skin (3 animals for each condition)

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 2 mL/kg bw
Remarks on result:
other: no animals died within the 14 day observation period

Any other information on results incl. tables

- no deaths occurred

- body weight development was not impaired

Applicant's summary and conclusion

Conclusions:
Single application of 2 mL of test substance per kg bw onto the intact or abraded skin of rabbits did not cause lethality during the 14 days observation period, thus resulting in a LD50 > 2 mL/kg bw. This study was judged not to be reliable, because relevant information (e.g. test item purity, duration of exposure) are missing. Nevertheless, these data support the results obtained with other members of this category, which did not show acute dermal toxicity, too.
Executive summary:

6 Rabbits were subjected to test acute dermal toxicity. The test substance was administered on the skin

(3 animals had intact skin, 3 animals had abraded skin) at a dose of 2 mL/kg bw. No animal died within the observation period, resulting in a LD50 > 2 mL/kg bw.