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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
Oct. 4, 1989-Oct. 18, 1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
EC Number:
270-115-0
EC Name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
Cas Number:
68411-30-3
Molecular formula:
C16-19 H25-31 Na O3 S
IUPAC Name:
sodium 4-undecylbenzenesulfonate

Sampling and analysis

Details on sampling:
Samples were from the 250 mg/kg dw concentration taken on day 0, 7, and 14. Measured concentrations were 235, 211, and 40.5 mg/kg dw respectively.

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
Test material was mixed with 1.16 L of deionized water, and then added to 2.44 kg of artificial soil.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Earthworm
- Source: Vittor and Associates, Mobile Alabama
- Age at test initiation (mean and range, SD): adult
- Weight at test initiation (mean and range, SD): average 0.66 g

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Total exposure duration:
14 d

Test conditions

Test temperature:
19-22 degrees C
pH:
5.5-5.8
Moisture:
34-36%
Details on test conditions:
TEST SYSTEM
- Test container (material, size): jars with perforated lids
- Amount of soil or substrate: 900 g moist soil
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil)
- % sand: 70
- % clay: 20, also 10% sphagnum peat
- Soil classification system: sandy loam


OTHER TEST CONDITIONS
- Photoperiod: constant lighting
- Light intensity: ~600 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, health, behaviour, and number of cocoons on days 7 and 14

Nominal and measured concentrations:
Nominal: 0, 63, 125, 250, 500, and 1000 mg/kg dw

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body weight
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
EC10
Effect conc.:
277 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: body weight
Remarks on result:
other: EC10 was not reported in original study report, but later calculated using study results using the method of Vanewijk, PH, and Hoekstra, JA, 1993. Calculation of the EC50 and its confidence interval when subtoxic stimulus is present. Ecotoxicol. Environ
Details on results:
There was 5% mortality at the 1000 mg/kg dw concentration on day 14. Body weight was decreased in the 500 and 1000 mg/kg dw groups by 23 and 33% respectively. The 1000 mg/kg dw group also showed lethargy and burrowing was also affected. Cocoon production was diminished in the 250 mg/kg dw group, and inhibited at higher concentrations.

Any other information on results incl. tables

Mortality and Weight Loss of Earthworms

Concentration

Number of

% Weight Loss

(mg/kg dw)

Day 0

Day 7

Day 14

Day 0-14

Control

40

40

40

14

63

40

40

40

5

125

40

40

40

6

250

40

40

40

5

500

40

40

40

23

1000

40

38

37

33

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 14-day LC50 to earthworms was > 1000 mg/kg dw soil. The 14-day NOEC was 250 mg/kg dw soil.
Executive summary:

Groups of earthworms were exposed to concentrations of 0, 63, 125, 250, 500, and 1000 mg/kg dw for 14 days. The test animals were examined for mortality, behaviour, health, and reproduction on days 7 and 14. 5% mortality was seen at the highest concentration. Effects to health were seen at the 500 and 1000 mg/kg dw concentrations. The 14 -day LC50 was > 1000 mg/kg dw, and the NOEC was 250 mg/kg dw.

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