Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with triethanolamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: APHA method (1980)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

triethanolamine

IUPAC Name:

triethanolamine

Constituent 2

Reference substance name:

2,2',2''-nitrilotriethanol

EC Number:

203-049-8

EC Name:

2,2',2''-nitrilotriethanol

Cas Number:

102-71-6

Molecular formula:

C6H15NO3

IUPAC Name:

2,2',2''-nitrilotriethanol

Details on test material:

- Name of test material (as cited in study report): Triethanolamine
- Analytical purity: 97 %

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- concentrations were analysed by gas-liquid chromatography (GLC)

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

Test species: Fathead minnow (Pimephales promelas)      
- age (d): 30       
- mean body length (mm): 18.1 ± 0.788      
- mean weight (g): 0.083 ± 0.0083      
- loading (g/l): 0.1153

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

25.7 ± 0.38 °C

pH:

7.8 ± 0.07

Dissolved oxygen:

7.3 mg/l

Nominal and measured concentrations:

Nominal test concentrations (g/l): 0, 7.30, 11.2, 17.3, 26.6, and 40.9
test substance recovery by GLC: 107.2 ± 3.8% (n = 4)

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 fish/concentration
- tank volume (l): 1.0 (additions 14.4 v/d)
- pH: the pH of the stock solution (90.4 mg/l) was adjusted to that of lake water using HCl.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

11 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The estimated LC/EC50 values with corresponding 95% confidence intervals were calculated using the corrected average of the analyzed tank concentrations and the Trimmed Spearman-Kaerber method (Hamilton et al., 1977).

Any other information on results incl. tables

Sublethal observations / clinical signs:

Effect values (related to measured concentrations): 96-h EC50 (g/l): 11.8 (10.6-13.0)

Mortalities

Nominal conc (g/l)	Mean measured (corr.)	No. of fish at start	Dead fish after
			24h	48h	72h	96h
Control	< 0.02	20	0	0	0	0
7.30	6.14	20	0	0	0	0
11.2	9.86	20	1	3	4	4
17.3	15.4	20	15	17	18	18
26.6	24.4	20	20	20	20	20
40.9	38.1	20	20	20	20	20

Physiological/behavioral effects 
Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 was 11800 mg/L based on measured concentrations.

Executive summary:

Acute toxicity of TEA to fathead minnows was tested according to APHA method (1980). No mortaility was observed in the control.

Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.

Effect values (related to measured concentrations): 96-h LC50 (g/l): 11.8 (10.6-13.0)