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EC number: 939-464-2 | CAS number: 121617-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From Mar. 07, 1983 to Aug. 12, 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
- Reference Type:
- publication
- Title:
- Predicted no-effect concentrations and risk characterization of four surfactants: Linear alkylbenzene sulfonate, alcohol ethoxylates, alcohol ethoxylated sulfates, and soap.
- Author:
- van de Plassche, E.J., de Bruijn, J.H.M., Stephenson, R.R., Marshall, S.J., Feijtel, T.C.J. and Belanger, S.E.
- Year:
- 1 999
- Bibliographic source:
- Environmental Toxicology and Chemistry. 18:2653-2663.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Comotto (1982), and Mount & Norberg (1983)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
Test material
- Reference substance name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- EC Number:
- 270-115-0
- EC Name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- Cas Number:
- 68411-30-3
- Molecular formula:
- C16-19 H25-31 Na O3 S
- IUPAC Name:
- sodium 4-undecylbenzenesulfonate
- Details on test material:
- - Name of test material: C11.8 Linear alkyl benzene sulfonate (LAS) (TSIN # X0029.01)
- Substance type: Pure active substance
- Physical state: Opaque paste
- Stability under test conditions: Not reported
- Storage condition of test material: At room temperature in a sealed container
- Other:
- Molecular weight: 345
- Solubility: freely water soluble
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- N/A
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Test solutions were prepared by adding the test material from a stock solution, prepared in deionized water, to the test water.
Test organisms
- Test organisms (species):
- Ceriodaphnia sp.
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Not reported
- Justification for species other than prescribed by test guideline: The standard chronic invert test uses Daphnia magna, and is 21 days. Ceriodaphnia, another water flea, is an alternate to Daphnia magna. The Ceriodaphnia chronic test is 7 days. Ceriodaphnia is similar in sensitivity to Daphnia.
- Source: Not reported
- Age of parental stock: Neonate Ceriodaphnia (¿24 h old)
- Feeding during test: Yes
- Food type: Diet of baker's yeast, or a mixture of algae, trout chow and alfalfa.
- Amount: Feeding amount was same as of stock cultures
- Frequency: Feeding frequency was same as of stock cultures
ACCLIMATION
- Acclimation period: Acclimated to test conditions (water/diet) combination for at least two generations.
- Acclimation conditions: Same as test
- Type and amount of food: Type of food was same as used during test
- Feeding frequency: Not reported
- Health during acclimation (any mortality observed): Not reported
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Not reported
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Average total hardness: 110± 9 mg/L as CaCO3
- Test temperature:
- Temperature was same as described for stock cultures
- pH:
- Average pH: 7.4±0.2
- Dissolved oxygen:
- Average dissolved oxygen: 9.7±0.8 mg/L
- Salinity:
- N/A
- Nominal and measured concentrations:
- Nominal: 0, 0.5, 1, 2, 3.5, 5, 7 mg a.i./L
- Details on test conditions:
- TEST SYSTEM
Test vessel: 50 mL beaker
- Type: Open
- Material: 50 mL glass beaker containing 30 mL of test solution
- Aeration: No (static-renewal test)
- Renewal rate of test solution: all test chambers were renewed with fresh test solution three times during the test procedure (on Day 2, 4 and 6)
- No. of organisms per vessel: One
- No. of vessels per concentration (replicates): Ten
- No. of vessels per control (replicates): Ten
- Biomass loading rate: 1 daphnid/30 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ohio River water
- Total suspended solids: 87 mg/L
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals: Not reported
- Pesticides: Not reported
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: Not reported
- Conductivity: Not reported
- Culture medium different from test medium: No
- Intervals of water quality measurement: pH and dissolved oxygen content of fresh and used test solutions were routinely monitored.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Same as of stock cultures
- Light intensity: Same as of stock cultures
EFFECT PARAMETERS MEASURED: Percent mortality, total reproduction, first day of reproduction, reproduction/individual, # of broods, brood size. All parameters were measured daily.
RANGE-FINDING STUDY: No
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 5.34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: CL=4.3-7.6; Ceriodaphnia were fed the yeast diet
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 5 - < 7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: Ceriodaphnia were fed the algae-trout chow diet
- Key result
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks:
- brood size
- Remarks on result:
- other: Ceriodaphnia were fed the yeast diet
- Key result
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: Ceriodaphnia were fed the algae-trout chow diet
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Ceriodaphnia were fed the yeast diet
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: Ceriodaphnia were fed the algae-trout chow diet
- Details on results:
- - Mortality of parent animals: For details on mortality, refer to `Table 1¿ and `Table 2¿ under 'Any other information on results incl. tables' section.
- No. of offspring produced per day per female: See Tables 1,2 below
- Type and number of morphological abnormalities: Not reported
- Type and number of behavioural abnormalities: Not reported
- Number of males and females (parental): Not reported
- Time to first brood release or time to hatch: 4-5 days in control
- Egg development time: Not reported
- Brood size: Please refer Table 1 and 2 under 'Any other information on results incl. table' section
- Time to sexual maturity: Not reported
- Type and magnitude of biochemical changes: Not reported
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- - Probit analysis was used to calculate LC50 values and associated 95 % confidence interval.
- Chi-square techniques was used for survival analysis.
- ANOVA technique was used for reproduction parameters.
Any other information on results incl. tables
Table 1. Chronic toxicity of LAS to Ceriodaphnia Sp.: Ohio River Water, yeast Diet (Study # 29717)
Concentration |
Total reproduction (per 10 daphnids) |
Percent mortality |
First day of reproduction |
Reproduction per daphnid |
# of Broods |
Brood size |
0 |
229 |
0 |
4.0 |
22.9 |
2.9 |
8.0 |
0.5 |
208@ |
0 |
3.9 |
20.8 |
2.9 |
7.3 |
1.0 |
178 |
0 |
4.0 |
17.8 |
2.9 |
6.1* |
2.0 |
145 |
10 |
4.0 |
16.1* |
3.0 |
5.4* |
3.5 |
78 |
10 |
4.4 |
8.7* |
2.4 |
3.4* |
5.0 |
8 |
10 |
6.6* |
0.9* |
0.6* |
1.8* |
7.0 |
0 |
100* |
- |
- |
- |
- |
* Significantly different from control (P<0.05)
@ Adjusted from 166 (n=8) to 208 (n=10)
Table 2. Chronic toxicity of LAS to Ceriodaphnia Sp.: Ohio River Water, algae-TC Diet (Study # 29717)
Concentration |
Total reproduction (per 10 daphnids) |
Percent mortality |
First day of reproduction |
Reproduction per daphnid |
# of Broods |
Brood size |
0 |
87 |
10 |
4.7 |
9.7 |
2.4 |
4.0 |
0.5 |
69 |
0 |
5.0 |
6.9 |
2.1 |
3.4 |
1.0 |
32 |
10 |
5.6* |
3.6* |
1.4* |
2.4* |
2.0 |
42 |
0 |
5.0 |
4.2* |
1.8 |
2.2* |
3.5 |
82 |
0 |
5.6* |
8.2 |
2.0 |
4.2 |
5.0 |
104 |
0 |
4.7 |
11.6 |
2.3 |
4.9 |
7.0 |
15 |
100* |
4.0 |
5.0* |
- |
5.0 |
* Significantly different from control (P<0.05)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The effect of C11.8 LAS (linear alkylbenzene sulfonate) on Ceriodaphnia was evaluated in a 7-day chronic toxicity test in river water. The NOEC, based on mortality and reproduction, was 0.5 mg/L and 5.0 mg/L for Ceriodaphnia fed a yeast or algae/trout chow diet, respectively. Results are based on nominal concentration of test substance (active ingredient).
- Executive summary:
A 7-day chronic toxicity test of linear alkylbenzene sulfonate (C11.8 LAS) was conducted with Ceriodaphnia sp under semi-static conditions. The test medium was Ohio River water, and the Ceriodaphnia were fed either a yeast diet, or an algae/trout chow diet. The test medium was renewed 3x/7 days.
The nominal test concentrations were 0, 0.5, 1, 2, 3.5, 5 and 7 mg/L of active ingredient. Mortality and reproduction were monitored daily. Results are based on the nominal concentration of the test substance. The 7 -day LC50 values were 5 -7 mg/L for both diets.
The 7 -day chronic NOECs may be summarized as follows:
Diet NOEC (mg/L) Based on
Yeast 0.5 Reproduction
Algae-Trout chow 5.0 Mortality and Reproduction
The chronic toxicity of LAS, in this 7 -day Ceriodaphnia test, compared quite favorably with the chronic toxicity of LAS in 21-day Daphnia magna tests. The data are for a C11.8 LAS and when normalized to a C11.6 LAS, the NOEC is 0.59 mg/L (method: van de Plassche et al., 1999).
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