Registration Dossier
Registration Dossier
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EC number: 939-464-2 | CAS number: 121617-08-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- June 7, 1984-June 22, 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-600/9-78-018
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- EC Number:
- 270-115-0
- EC Name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- Cas Number:
- 68411-30-3
- Molecular formula:
- C16-19 H25-31 Na O3 S
- IUPAC Name:
- sodium 4-undecylbenzenesulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- no information
Test solutions
- Details on test solutions:
- A stock solution of 1,000 mg product/1000 ml DI water was prepared. From this, a second stock solution was prepared with a dilution factor of 20.
Test organisms
- Test organisms (species):
- other: Chlorella kessleri
Study design
- Test type:
- static
- Water media type:
- not specified
- Total exposure duration:
- 15 d
Test conditions
- Nominal and measured concentrations:
- 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 100 ml DI water, and 10 ml mineral medium
- Initial cells density: 10^4 cells/ml
- Control end cells density: 3.7 x 10^15
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, AAP-Medium
OTHER TEST CONDITIONS
- Light intensity and quality: ~2000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of cells was determined on days 5, 7, 12, and 15
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 15 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
- Duration:
- 15 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- cell number
Any other information on results incl. tables
Number of cells/ml (average of 3 replicates)
Concentration (mg/L) |
5 days |
7 days |
12 days |
15 days |
0 |
7.2 x 105 |
1.5 x 106 |
3.9 x 106 |
3.7 x 106 |
0.01 |
5.9 x 105 |
1.4 x 106 |
2.4 x 106 |
3.2 x 106 |
0.03 |
3.0 x 105 |
1.4 x 106 |
3.4 x 106 |
3.6 x 106 |
0.1 |
2.6 x 105 |
1.3 x 106 |
2.4 x 106 |
3.0 x 106 |
0.3 |
2.5 x 105 |
1.3 x 106 |
3.2 x 106 |
3.5 x 106 |
1 |
2.6 x 105 |
1.3 x 106 |
2.5 x 106 |
2.8 x 106 |
3 |
1.6 x 105 |
1.0 x 106 |
2.3 x 106 |
3.2 x 106 |
10 |
3.9 x 105 |
6.3 x 105 |
1.5 x 106 |
1.7 x 106 |
30 |
1.9 x 105 |
3.9 x 105 |
1.1 x 106 |
1.4 x 106 |
100 |
2.9 x 104 |
4.5 x 104 |
4.2 x 104 |
6.1 x 104 |
300 |
1.3 x 104 |
2.7 x 104 |
1.9 x 104 |
1.1 x 104 |
1000 |
1.6 x 104 |
2.6 x 104 |
2.5 x 104 |
2.0 x 104 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC for algae was 3.1 mg/L (active ingredient), and the LOEC was 10 mg/L (active ingredient).
- Executive summary:
Chlorella kessleri algae was exposed to concentrations of 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, and 1000 mg/L (nominal) of test substance for 15 days. The number of cells were counted to determine the toxicity of the test substance. The NOEC was 3.1 mg/L active ingredient, and the LOEC was 10 mg/L active ingredient. The data are for C11.6 LAS and no normalization is required.
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