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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
June 7, 1984-June 22, 1984
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EPA-600/9-78-018
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
EC Number:
270-115-0
EC Name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
Cas Number:
68411-30-3
Molecular formula:
C16-19 H25-31 Na O3 S
IUPAC Name:
sodium 4-undecylbenzenesulfonate

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no information

Test solutions

Details on test solutions:
A stock solution of 1,000 mg product/1000 ml DI water was prepared. From this, a second stock solution was prepared with a dilution factor of 20.

Test organisms

Test organisms (species):
other: Chlorella kessleri

Study design

Test type:
static
Water media type:
not specified
Total exposure duration:
15 d

Test conditions

Nominal and measured concentrations:
0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: 100 ml DI water, and 10 ml mineral medium
- Initial cells density: 10^4 cells/ml
- Control end cells density: 3.7 x 10^15
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, AAP-Medium


OTHER TEST CONDITIONS
- Light intensity and quality: ~2000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of cells was determined on days 5, 7, 12, and 15

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
15 d
Dose descriptor:
NOEC
Effect conc.:
3.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Duration:
15 d
Dose descriptor:
LOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number

Any other information on results incl. tables

Number of cells/ml (average of 3 replicates)

Concentration (mg/L)

5 days

7 days

12 days

15 days

0

7.2 x 105

1.5 x 106

3.9 x 106

3.7 x 106

0.01

5.9 x 105

1.4 x 106

2.4 x 106

3.2 x 106

0.03

3.0 x 105

1.4 x 106

3.4 x 106

3.6 x 106

0.1

2.6 x 105

1.3 x 106

2.4 x 106

3.0 x 106

0.3

2.5 x 105

1.3 x 106

3.2 x 106

3.5 x 106

1

2.6 x 105

1.3 x 106

2.5 x 106

2.8 x 106

3

1.6 x 105

1.0 x 106

2.3 x 106

3.2 x 106

10

3.9 x 105

6.3 x 105

1.5 x 106

1.7 x 106

30

1.9 x 105

3.9 x 105

1.1 x 106

1.4 x 106

100

2.9 x 104

4.5 x 104

4.2 x 104

6.1 x 104

300

1.3 x 104

2.7 x 104

1.9 x 104

1.1 x 104

1000

1.6 x 104

2.6 x 104

2.5 x 104

2.0 x 104

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC for algae was 3.1 mg/L (active ingredient), and the LOEC was 10 mg/L (active ingredient).
Executive summary:

Chlorella kessleri algae was exposed to concentrations of 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, and 1000 mg/L (nominal) of test substance for 15 days. The number of cells were counted to determine the toxicity of the test substance. The NOEC was 3.1 mg/L active ingredient, and the LOEC was 10 mg/L active ingredient. The data are for C11.6 LAS and no normalization is required.

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