Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with triethanolamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

June 7, 1984-June 22, 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-600/9-78-018

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

C16-19 H25-31 Na O3 S

IUPAC Name:

sodium 4-undecylbenzenesulfonate

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

no information

Test solutions

Details on test solutions:

A stock solution of 1,000 mg product/1000 ml DI water was prepared. From this, a second stock solution was prepared with a dilution factor of 20.

Test organisms

Test organisms (species):

other: Chlorella kessleri

Study design

Test type:

static

Water media type:

not specified

Total exposure duration:

15 d

Test conditions

Nominal and measured concentrations:

0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 100 ml DI water, and 10 ml mineral medium
- Initial cells density: 10^4 cells/ml
- Control end cells density: 3.7 x 10^15
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, AAP-Medium


OTHER TEST CONDITIONS
- Light intensity and quality: ~2000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of cells was determined on days 5, 7, 12, and 15

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

15 d

Dose descriptor:

NOEC

Effect conc.:

3.1 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

cell number

Effect concentrations 2

Duration:

15 d

Dose descriptor:

LOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

cell number

Any other information on results incl. tables

Number of cells/ml (average of 3 replicates)

Concentration (mg/L)	5 days	7 days	12 days	15 days
0	7.2 x 10^5	1.5 x 10^6	3.9 x 10^6	3.7 x 10^6
0.01	5.9 x 10^5	1.4 x 10^6	2.4 x 10^6	3.2 x 10^6
0.03	3.0 x 10^5	1.4 x 10^6	3.4 x 10^6	3.6 x 10^6
0.1	2.6 x 10^5	1.3 x 10^6	2.4 x 10^6	3.0 x 10^6
0.3	2.5 x 10^5	1.3 x 10^6	3.2 x 10^6	3.5 x 10^6
1	2.6 x 10^5	1.3 x 10^6	2.5 x 10^6	2.8 x 10^6
3	1.6 x 10^5	1.0 x 10^6	2.3 x 10^6	3.2 x 10^6
10	3.9 x 10^5	6.3 x 10^5	1.5 x 10^6	1.7 x 10^6
30	1.9 x 10^5	3.9 x 10^5	1.1 x 10^6	1.4 x 10^6
100	2.9 x 10^4	4.5 x 10^4	4.2 x 10^4	6.1 x 10^4
300	1.3 x 10^4	2.7 x 10^4	1.9 x 10^4	1.1 x 10^4
1000	1.6 x 10^4	2.6 x 10^4	2.5 x 10^4	2.0 x 10^4

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC for algae was 3.1 mg/L (active ingredient), and the LOEC was 10 mg/L (active ingredient).

Executive summary:

Chlorella kessleri algae was exposed to concentrations of 0, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 300, and 1000 mg/L (nominal) of test substance for 15 days. The number of cells were counted to determine the toxicity of the test substance. The NOEC was 3.1 mg/L active ingredient, and the LOEC was 10 mg/L active ingredient. The data are for C11.6 LAS and no normalization is required.