Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Remarks:
The test conditions are well described and are in accordance with the recognised EC and OECD test guidelines. The GLP are not mentioned.

Data source

Reference
Reference Type:
publication
Title:
Acute toxicities of organic chemicals to fathead minnow (Pimephales promelas)
Author:
Brooke LT , Call DJ, Geiger DL and Northcott CE
Year:
1984
Bibliographic source:
Center for lake Superior Environmental Studies 1984, vol I: 327-328

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
3-ethoxy-4-hydroxybenzaldehyde
EC Number:
204-464-7
EC Name:
3-ethoxy-4-hydroxybenzaldehyde
Cas Number:
121-32-4
Molecular formula:
C9H10O3
IUPAC Name:
3-ethoxy-4-hydroxybenzaldehyde
Details on test material:
from Aldrich Chemicals Co
Purity : 97 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Toxicants were proportionally diluted with lake superior water from stock solution before delivery to fish exposure chamber.
Test chemicals were prepared for proportional dilution by the diluted stock method. i e : premixed aqueous solution of desired concentration.
Stock solution: 1000 mg/L
- Controls: blank

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Pimephales promelas (Fathead minnow)
- Source: From US EPA Environmental Research Laboratory- Duluth
- Age at study initiation: 28 days
- Weight at study initiation: Mean: 0.034 g; SD: 0.0131 g
- Length at study initiation: Mean: 14.3 mm; SD: 2.245 mm
- Method of breeding: Adults were held at 25°C in flowing water with a 16-h light-controlled photoperiod and fed frozen adult brine shrimp. They were provided with asbestos pipe (cut in half longitudinally) as spawning substrate. Eggs were spawed and fertilized on the undersides of these substrates. The embryos were tended by the male parent until eye-up at which time the substrates with embryos attached were removed to another 25°C bath where hatching occured.
- Feeding during test: no

ACCLIMATION
- Acclimation period: no

QUARANTINE: no data

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
behaviour also reported during the test

Test conditions

Hardness:
52.2 mg/L CaCO3 (SD: 0.65) measured by the compleximetric or EDTA method
Test temperature:
24.1 °C (SD: 0.83) measured daily using partial immersion of a mercury thermometer
pH:
7.69 (SD: 0.28) measured with a meter calibrated shortly prior each test
Dissolved oxygen:
6.5 mg/L (SD: 0.41) measured with an oxygen sensitive electrode calibrated weekly using the azide modification of the winkley method
Salinity:
no data (fresh water)
Nominal and measured concentrations:
nominal concentration : 0 / 12.5 / 25 / 50 / 100 / 200 /
measured concentrations : see table 1 in "any other information"
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 3.4 l glass open tank
- Aeration: no
- Type of flow-through: cycling proportional diluters
- Renewal rate of test solution: no data
- No. of organisms per vessel: 22 to 26 fish per tank
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 0

WATER PARAMETERS:
- Source: Lake Superior water
- Total organic carbon:
- Metals: Al (1-26 µg/L); Cd (<0.1 µg/L); Cr (2-20 µg/L); Co (<0.5 µg/L); Cu (0.3-3.2 µg/L); Fe (2-83 µg/L); Pb (7-20 µg/L); Mn (0.2-11.5 µg/L); Ni (<0.5 µg/L); Zn (1-2.7 µg/L)
- Intervals of water quality measurement: at least once for each test.

TEST MEDIUM PARAMETERS:
- Test temperature: 24.1 °C
- dissolved oxygen: 6.5 mg/l
- hardness (CaCO3): 52.5 mg/L
- pH: 7.69

OTHER TEST CONDITIONS: no data
photoperiod : no data

EFFECT PARAMETERS MEASURED: mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 0, 12.5, 25, 50, 100 and 200 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
87.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 81.4 - 94.3
Details on results:
Affected fish swan at surface. Respiration and gill movements were limited. Fish were also hypoactive and lost equilibrium prior to death.
Results with reference substance (positive control):
not relevant
Reported statistics and error estimates:
no data

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 2: Mortalities observed in each exposure chamber

Hour

control

A

B

C

D

E

Initial n° of fish in each exposure chamber

26

25

25

25

23

22

26

24

25

26

25

25

1.75

1

0

0

1

1

0

1

0

0

1

3

2

3

1

0

0

1

1

0

1

0

0

1

3

4

6

1

0

0

1

1

0

1

0

0

1

4

6

12

1

0

0

1

1

0

2

0

0

1

21

20

24

1

0

0

1

2

0

3

0

0

1

22

22

29.5

1

0

0

1

2

0

3

0

0

1

22

22

48

1

0

0

1

2

0

3

0

3

1

24

24

53.5

1

0

0

1

2

0

3

0

3

1

24

24

72

1

0

0

1

2

0

3

0

3

1

25

25

77.2

1

0

0

1

2

0

3

0

3

1

25

25

96

1

0

0

1

2

1

3

0

3

1

25

25

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
With an EC50-96h = 87.6 mg/L for Pimephales promelas, ethylvanillin is harmful for fish.
The validity criteria were not examined by the authors but 3 of the 4 validity criteria detailled in 203 OECD guideline were fullfilled:
- mortality in the controls did not exceed 10% at the end of the test
- the test was conducted under flow through conditions
- deviation of measured concentration from nominal ones was greater than 20% BUT the results were based on measured concentrations.

Concerning oxygen concentration, it is described that "test mean concentration were maintained between 60 and 100 % of water saturation except in 12 tests" it is not clear if it is the case for ethylvanillin test.
Executive summary:

In a 96-h acute toxicity study (Brooke, 1984), Fathead Minnows (Pimephales promelas) were exposed to ethyl vanillin at nominal concentrations of 0 (control); 12.5; 25; 50; 100 & 200 mg./L under flow through conditions. 

The 96-h LC50 was 87.6 mg a.i./L.  95% C.I.:  81.4 to 94.3 mg./L (based on measured concentrations)

According to directive 67/548/CEE, Ethylvanillin is considered as harmful for Pimephales promelas in the conditions tested.

Although GLP were not mentionned and validity criteria not detailled, this toxicity study satisfies the guideline requirement and is therefore considered as reliable with restrictions.

Categories Display