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EC number: 204-464-7 | CAS number: 121-32-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Study not performed according to actual standardised guideline, but method scientifically sounds.
Data source
Reference
- Reference Type:
- publication
- Title:
- A rapid seedling bioassay for the study of allelopathy
- Author:
- Shilling D & Yoshikawa F
- Year:
- 1 987
- Bibliographic source:
- American Chemical Society (Ed.), chapter 31: 334-342
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Phytotoxic activity of 26 compounds (including vanillin and ethylvanillin) was determined by measuring different growth parameters using two test species: Echinochloa crusgalli L. Beauvois (barnyardgrass, a monocot) and Sesbania exaltata Raf. Cory. (hemp sesbania, a dicot).
Growth parameters followed were :
•Shoot length (i.e. coleoptile and leaf tissue for barnyardgrass and hypocotyl tissue for hemp sesbania)
•Root length
•Total fresh weight
Seeds were placed on a filter paper, in jars, with the test substance and MES buffer. Seeds were let germinated at 25°C with a 12h photoperiod and harvested after 60h and 72h for barnyardgrass and hemp sesbania respectively. - GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-ethoxy-4-hydroxybenzaldehyde
- EC Number:
- 204-464-7
- EC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Cas Number:
- 121-32-4
- Molecular formula:
- C9H10O3
- IUPAC Name:
- 3-ethoxy-4-hydroxybenzaldehyde
- Details on test material:
- no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Ethylvanillin was dissolved in acetone and the 2 mM solution was placed in 60 jars with Whatman #1 filter paper. After the solvent was evaporated, either 0.22 g (60 seeds) of barnyardgrass or 0.40 g (30 seeds) of hemp sesbania were placed in jars. 3 mL and 3.5 mL of 15 mM MES buffer [2-morpholinoethanesulfonic acid] adjusted to pH 6.0 was added for barnyardgrass and hemp sesbania respectively.
- Controls: acetone-treated filter paper alone
- Chemical name of vehicle: acetone
- Concentration of vehicle in test medium (stock solution and final test solution): no data
- Evaporation of vehicle before use: yes
Test organisms
open allclose all
- Species:
- other: Echinochloa crusgalli L. Beauvois
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Common name: barnyardgrass
- Species:
- other: Sesbania exaltata Raf. Cory.
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Common name: hemp sesbania
Study design
- Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- filter paper
- Total exposure duration:
- 72 h
- Remarks:
- Barnyardgrass harvested after 72h of exposition and hemp sesbania after 60h
Test conditions
- Test temperature:
- 25°C
- pH:
- 6.0
- Details on test conditions:
- seed germination/root elongation toxicity test: rapid seedling bioassay.
TEST SYSTEM
- Test container (type, material, size): 60 mL jars
- Method of seeding: seeds were put upon the paper filter
- No. of seeds per container: 0.22 g (60 seeds) of barnyardgrass // 0.40 g (30 seeds) of hemp sesbania
- No. of replicates per treatment group: no data
- No. of replicates per control: no data
- No. of replicates per vehicle control: no data
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
NUTRIENT MEDIUM (if used)
- Description: MES buffer [2-morpholinoethanesulfonic acid]
GROWTH CONDITIONS
- Photoperiod: 12h
- Light source: no data
- Light intensity and quality: no data
- Day/night temperatures: 25°C
- Water source/type: batch culture
- Volume applied: 3 mL (barnyardgrass) and 3.5 mL (hemp sesbania)
- Method of application: no data
ACCLIMATION PERIOD: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : no data
VEHICLE CONTROL PERFORMED: no data - Nominal and measured concentrations:
- 2mM (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- alpha-phenyllactic acid and p-ethoxybenzoic acid
Results and discussion
Effect concentrations
- Key result
- Species:
- other: Echinochloa crusgalli L. Beauvois (barnyardgrass)
- Duration:
- 72 h
- Dose descriptor:
- other: 4 growth parameters : shoot length / root length / total fresh weight / Predicted shoot plus root fresh weight
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks on result:
- other: Only the total fresh weight is significantly different from the control at 2mM.
- Details on results:
- SEED GERMINATION
- Percent seed germination versus control plants:
barnyardgrass: 93%
hemp sesbania: 89%
see different growth parameters in table 1 - Results with reference substance (positive control):
- see table 1
- Results with reference substance valid? yes
Any other information on results incl. tables
Table 1: Percentage of inhibition of the compounds on four growth parameters of banyardgrass and hemp sesbania.
Chemical |
Barnyardgrass |
Hemp sesbania |
||||
Shoot lenght |
Root lenght |
Total fresh weight |
Shoot lenght |
Root lenght |
Total fresh weight |
|
a-phenyllactic acid (positive control) |
12 |
57* |
22* |
13* |
56* |
22* |
p-ethoxybenzoic acid (positive control) |
18* |
84* |
29* |
22* |
66* |
27* |
Ethyl vanillin |
15 |
17 |
15* |
0 |
0 |
0 |
Values followed by an asterisk are significantly different from the control at the 0.05 level according to general linear model procedure.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In the tested conditions, no or slight inhibition on plant growth factor was observed (shoot length / root length / total fresh weight)
- Executive summary:
In a study (Shilling et al., 1987), the toxicity to terresterial plants was evaluated. The observations were the following:
Hemp sesbania: 0% of inhibition is observed for all growth parameter followed when seeds are exposed to ethyl vanillin at 2 mM during 60h. In the test conditions, Ethylvanillin as no phytotoxic effect on this species.
Barnyardgrass: Only a slight inhibition (between 15 and 19%) on growth parameters has been observed after 72h of exposition of ethylvanillin at 2 mM. But it is significantly different from the controls only for 1 of the 3 growth parameters (total fresh weight) but not for the most sensitive one the root length.
Only the total fresh weight is significantly different from the control. The two other parameters measured (root and shoot lengths) are not significantly different from the control. It is may be due to the difficulty on measuring these parameters. But authors also indicate that root growth was the most sensitive parameter and total fresh weight even if easier to measure is less sensitive. As Ethyl vanillin showed an effect only on the less sensitive growth parameter, and not on the two other more sensitive ones, it is therefore reasonnable to assess that Ethyl vanillin as no phytotoxic effect on this species in the described conditions.
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