Registration Dossier

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Some details are missing and no data/response relationship.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1995

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
2,4-diaminotoluene
ex Aldrich Chemical Co Inc, Milwaukee, WI, USA.
Purity 98%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Twice a week during the 4 weeks study.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
Larvae (0-3 days old) used.

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d

Test conditions

Hardness:
mean 45.8 mg/l as CaCO3
Test temperature:
25 +/- 1°C
pH:
mean 7.88 +/- 0.18
Dissolved oxygen:
mean 6.8 mg/l
range 5 - 8.5 mg/l
Nominal and measured concentrations:
Measured concentrations: mean values 0, 40.3, 68.8, 131, 263, 551
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Dose descriptor:
NOEC
Nominal / measured:
meas. (arithm. mean)
Basis for effect:
weight
Remarks on result:
other: could not be determined

Any other information on results incl. tables

At the lowest tested concentration of 40.3mg/l the weight of the larvae was significantly reduced, while survival was significantly reduced at 68.8mg/l. No NOEC could be derived from this test.

Applicant's summary and conclusion