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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Detailed GLP study carried out in accordance with OECD Guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
2,4-TDA
2,4-diaminotouene
Supplied by Tokyo Chemical Industry Co. Ltd
Purtiy 99.9%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Pagrus major
Details on test organisms:
Mean weight - 0.76g
Mean total length - 4.2cm
Supplier Fisheries Laboratory, Kinki University, Wakayama, Japan

Study design

Test type:
flow-through
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20 +/- 1°C
pH:
8.0 - 8.1
Dissolved oxygen:
6.3 - 7.3 mg/l
(>75% of saturated concentration at the water temperature)
Salinity:
Natural sea water
Nominal and measured concentrations:
0, 0.107, 0.222, 0.444, 0.849 and 1.98 mg/l
Details on test conditions:
Abnormal responses were recorded throughout the study.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1.98 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
1.78 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.414 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1.98 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.107 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion