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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Detailed GLP study carried out in accordance with OECD Guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methyl-m-phenylenediamine
EC Number:
202-453-1
EC Name:
4-methyl-m-phenylenediamine
Cas Number:
95-80-7
Molecular formula:
C7H10N2
IUPAC Name:
4-methylbenzene-1,3-diamine
Details on test material:
2,4-TDA
2,4-diaminotouene
Supplied by Tokyo Chemical Industry Co. Ltd
Purtiy 99.9%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Pagrus major
Details on test organisms:
Mean weight - 0.76g
Mean total length - 4.2cm
Supplier Fisheries Laboratory, Kinki University, Wakayama, Japan

Study design

Test type:
flow-through
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20 +/- 1°C
pH:
8.0 - 8.1
Dissolved oxygen:
6.3 - 7.3 mg/l
(>75% of saturated concentration at the water temperature)
Salinity:
Natural sea water
Nominal and measured concentrations:
0, 0.107, 0.222, 0.444, 0.849 and 1.98 mg/l
Details on test conditions:
Abnormal responses were recorded throughout the study.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1.98 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
1.78 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.414 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1.98 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.107 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion