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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: (R)-2-Chloromandelic acid
- purity: 99,7 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution, for the limit and range-finding tests, was prepared by dissolving the test item directly in LC reconstituted water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: CCAP 276/20.
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria, LA22 OLP, UK.
- Method of cultivation: the algae are cultured under sterile conditions and inaintained at exponential growth rate.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
24.1°C - 25.0°C
pH:
7.3-8.26
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass test vessels (250 mL Erlenmeyer flasks)
- Aeration: the cultures are constantly aerated with air filtered using a 0.22 porosity filter to maximize the CO2 availability (and thereby the cell growth) while maintaining the sterility.

- Initial cells density:
- Control end cells density: 1.0E+04 cells/mL

- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted-LC
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

RS-Freetext:
Effect values based on biomass:
-------------------------------
NOEbC (72 h): >/= 100 mg/L
EbC50 (72 h): > 100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes