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Environmental fate & pathways

Adsorption / desorption

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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- test substance: (R)-2-Chloromandelic acid
- purity: 99.5%
Radiolabelling:
no

Study design

Test temperature:
25°C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: Modular HPLC System with UV/vis detector.
- Type, material and dimension of analytical (guard) column: 250 x 3 mm Nucleosil 100-5 CN (Macherey & Nagel)
- Detection system: UV/vis detector


MOBILE PHASES
- Type: water 1 acetonitrile = 9:1 (v/v) with 0.1 vol% H3P04, pH = 2.2
- Experiments with additives carried out on separate columns: no data
- pH: 2.2
- Solutes for dissolving test and reference substances: no data

DETERMINATION OF DEAD TIME
- Method: The dead time of the HPLC-system was measured withformamide.

REFERENCE SUBSTANCES
- Identity: Acetanilide

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 40 mg of test item was weighed in a 100 ml volumetric flask and dissolved in water/acetonitrile = 9:1 (v/v). This solution was diluted 1:5 with waterlacetonitrile = 9:1 (v/v).
For calibration, small amounts of reference compounds were dissolved in 20 vol% acetonitrile (ultrasonic treatment) and the flask was made up to volume with water.

REPETITIONS
- Number of determinations: no data


Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
< 17.78
Type:
log Koc
Value:
< 1.25

Results: Batch equilibrium or other method

Transformation products:
not measured

Any other information on results incl. tables

RS-Freetext:
log Koc < acetanilide (1.25) at 25 °C

Applicant's summary and conclusion

Validity criteria fulfilled:
yes