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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: (R)-2-Chlormandelsäure
- purity: 99.7 %
- aggregate state: solid/crystalline
- colour: white up to slightly yellowish

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from laboratory scale waste water plants fed with municipal sewage
- Pretreatment: none
- Concentration of sludge: 30 mg/L dry substance
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
39.5 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 +/- 2°C
- pH: 7.4-7.7
- pH adjusted: no

TEST SYSTEM
- Culturing apparatus: glass bootles
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: every 2 - 4 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes



STATISTICAL METHODS:
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
0 - 10
Sampling time:
28 d
Details on results:
Kinetic of control substance (in %):
= 10 after 3 day(s)
= 89 after 5 day(s)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed