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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Test substance: (R)-2-Chloromandelic acid
- purity: 99,7 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance was dissolved directly in test water

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: CIT, animals were cultured from eggs obtained from Viviers de France, Piscicultures des Fontaines, 64490 Sarrance, France.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
13-17 °C
pH:
7.37-7.82
Details on test conditions:
TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1g fish per L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered and dechlorinated potable water which is then softened by a Permo® water softening system.Half of the water passes directly into a holding tank attached to the flow-through system while the remaining half passes through a dejonizer producing ultra-pure water which is then fed into the holding tank. This results in a 1:1 dilution of potable dechlorinated:deiofliZed water giving a supply of die desirable hardness. The pH of die solution is between 6.0 and 8.5 and die total hardness is 150 ± 20 mgIL as CaCO3. The typical quality characteristics of this water are presented in appendix 2.

OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Other biological observations: One fish died while the range finding test in the concentration 10 mg/L. Due tto the fact, that in the limit test afterwards, no deaths occured in the highest concentration of 100 mg/L it is assumed to be an accidental death (i.e. not due to the test item)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes