(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

EC Number:

444-040-0

EC Name:

(2R)-2-(2-chlorophenyl)-2-hydroxyethanoic acid

Cas Number:

52950-18-2

Molecular formula:

Hill formula: C8H7ClO3 CAS formula: C8H7CLO3

IUPAC Name:

(2R)-2-(2-chlorophenyl)-2-hydroxyacetic acid

Details on test material:

- Test substance: (R)-2-Chloromandelic acid
- purity: 99,7 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance was dissolved directly in test water

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: CIT, animals were cultured from eggs obtained from Viviers de France, Piscicultures des Fontaines, 64490 Sarrance, France.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

13-17 °C

pH:

7.37-7.82

Details on test conditions:

TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1g fish per L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered and dechlorinated potable water which is then softened by a Permo® water softening system.Half of the water passes directly into a holding tank attached to the flow-through system while the remaining half passes through a dejonizer producing ultra-pure water which is then fed into the holding tank. This results in a 1:1 dilution of potable dechlorinated:deiofliZed water giving a supply of die desirable hardness. The pH of die solution is between 6.0 and 8.5 and die total hardness is 150 ± 20 mgIL as CaCO3. The typical quality characteristics of this water are presented in appendix 2.

OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Other biological observations: One fish died while the range finding test in the concentration 10 mg/L. Due tto the fact, that in the limit test afterwards, no deaths occured in the highest concentration of 100 mg/L it is assumed to be an accidental death (i.e. not due to the test item)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes