3,4-dihydro-2H-pyran

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

110-87-2

IUPAC Name:

110-87-2

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Offenbach/Main, FRG
- Housing: singel housing
- Diet (e.g. ad libitum): about 130 g (Ovator Solikanin 4 MM, Muskator-Werke, Duesseldorf FRG)
- Water (e.g. ad libitum): about 250 ml tap water/day
- Acclimation period: about 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 -24
- Humidity (%): 30 -70
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:

not specified

Preparation of test site:

clipped

Vehicle:

unchanged (no vehicle)

Controls:

no

other: untreated skin sites of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml

Duration of treatment / exposure:

4 hours

Observation period:

up to 8 days

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with Lutrol and Lutrol/water (1:1)
- Time after start of exposure: at the end of exposure period

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24, 48, and 72 hrs

Score:

3

Max. score:

3

Reversibility:

not fully reversible within: 8 d

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24, 48, and 72 hrs

Score:

1.8

Max. score:

3

Reversibility:

fully reversible

Any other information on results incl. tables

Times of readings	Animal	Intact skin
		Erythema	Edema	Symptoms
4 h	1	3	3	R:E/ED:E
	2	3	3	R:E/ED:E
	3	3	3	R:E/ED:E
24 h	1	3	3	R:E/ED:E
	2	3	3	R:E/ED:E
	3	3	2	R:E/ED:E
48 h	1	3	1	R:E/ED:E
	2	3	2	R:E/ED:E
	3	3	1	R:E/ED:E
72 h	1	3	1	R:E/ED:E/HE
	2	3	2	R:E/ED:E/HE
	3	3	1	R:E/ED:E/HE
8 days	1	4	0	N:SU, PU/+
	2	1	0	S:SE/+
	3	4	0	N:SU,PU,SP/+

EXPLANATIONOFSYMPROMS:

HE : HEMORRHAGIC

N:SU,PU : NECROSIS                     SUPERFICIAL PUNCTIFORM

N:SP:NECROSIS                           SPOT-LIKE EXTENDING

E:E : EDEMA                             BEYOND THEAREAOF EXPOSURE

R:E : ERYTHEMA                        EXTENDING BEYOND THEAREA OFEXPOSURE

S:SE : SCALING                           SEVERE

+ FINDING CONFIRMED BY GROSS-PATHOLOGICALEXAMINATION

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

Based on the available data, the test substance showed irritative effects after application to the skin of rabbits.

Executive summary:

The undiluted test substance was droped at a test patch (2.5 x 2.5 cm). After adsorption the patch was placed on the upper third of the back or flanks. The untreated skin sites served as the negative control. The reading of the parameter was conducted 24, 48, 72 hours, and 8 days after removal of the test patches. Based on the results of the available data, the test substance showed irritative effects after application to the skin of rabbits.