N,N-bis(3-aminopropyl)methylamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP, no guideline followed but similar to the OECD 402 guideline with deviations.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

, less than 5 animals per dose (4 animals max. were used per dose). In the oldest study 2 dose levels were examined instead of 3 dose levels.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-bis(3-aminopropyl)methylamine

EC Number:

203-336-8

EC Name:

N,N-bis(3-aminopropyl)methylamine

Cas Number:

105-83-9

Molecular formula:

C7H19N3

IUPAC Name:

bis(3-aminopropyl)(methyl)amine

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 3 - 5 months
- Weight at study initiation: average 2.5 kg
- Diet: ad libitum, Rockland standard rabbit diet
- Water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 hours

Doses:

In the oldest study (1957), 2 doses were used, 0.10 mL/kg bw and 0.20 mL/kg bw of the undiluted test substance.
In the latest study (1974) 3 doses were used, 0.20 mL/kg bw, 0.40 mL/kg bw and 0.80 mL/kg bw of the undiluted test substance.

No. of animals per sex per dose:

In the oldest study (1957) 4 male animals per dose were examined.
in the latest study (1974) two dose groups consisted of 4 male animals per dose and one group with 2 animals (the group with the highest concentration examined)

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, indications on the skin, gross pathology

Statistics:

Thompson´s method of calculating LD50 was used.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

0.14 mL/kg bw

Based on:

test mat.

Remarks on result:

other: equivalent to LD50=127 mg/kg bw. The result was obtained in the oldest study (1957)

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

0.252 mL/kg bw

Based on:

test mat.

Remarks on result:

other: Equivalent to LD50=228 mg/kg bw. The result was obtained in the latest study (1974).

Mortality:

Oldest study (1957):
In the dose group 0.10 mL/kg bw no mortality was observed.
In the 0.20 mL/kg bw dose group mortality of 4/4 within 1 -3 days (100 % mortality)
Latest study (1974):
In the 0.20 mL/kg bw dose group mortality of 1/4
in the 0.40 mL/kg bw dose group mortality of 4/4 (100 % mortality)
In the 0.80 mL/kg bw dose group mortality of 2/2 (100 % mortality)

Clinical signs:

other: On the skin applied with the test substance marked skin necrosis was observed. Symptoms as unsteady gait and lethargic were indicated in the later study (1974).

Gross pathology:

Oldest study (1957): lung hemorrhage, pale or yellow coloration of livers, pale yellow kidneys with potential hemorrhage.
Latest study (1974): by animals that died during the exposure/ observation period livers and kidneys were pale and mottled , lungs and spleens congested. No gross pathological indications were observed by survivors.