N,N-bis(3-aminopropyl)methylamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979-06-20 until 1979-07-24

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP, no guideline was followed/ mentioned (but similar to OECD 401 guideline)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-bis(3-aminopropyl)methylamine

EC Number:

203-336-8

EC Name:

N,N-bis(3-aminopropyl)methylamine

Cas Number:

105-83-9

Molecular formula:

C7H19N3

IUPAC Name:

bis(3-aminopropyl)(methyl)amine

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: By males an average weight of 262.5 g, by females an average weight of 207.5 g
- Fasting period before study: 15 h - 20 h before administration
- Diet: Herilan MRH-Haltung ; H. Eggersmann KG

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 10.0, 6.81, 4.64, 3.16 % (w/v)
- Amount of vehicle (if gavage): 10 mL/kg

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg

Doses:

1000, 681, 464, 316 mg/kg

No. of animals per sex per dose:

5 animals per sex per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: For mortality -1hour, 1, 2, 7, 14 days, for weighing 2 - 4 days, 7, 13 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology

Statistics:

Chi - 2 test

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

691 mg/kg bw

Based on:

test mat.

Mortality:

At the dose group of 316 mg/kg bw 0 % mortality after 14 days of observation
At the dose group of 464 mg/kg bw 10 % mortality after 14 days of observation
At the dose group of 681 mg/kg bw 60.0 % mortality after 14 days of observation
At the dose group of 1000 mg/kg bw 80 % mortalityafter 14 days of observation.

Clinical signs:

other: At dose groups 464, 691 and 1000 mg/kg bw animals were in poor general state and the following signs and symptoms were observed: dyspnea, apathy, staggering, spastic gait, piloerection, erythema and salivation.

Gross pathology:

Necropsy findings by animals that died:
Heart: acute dilatation ; acute passive hyperemia
Liver: peripheral clay-colored acinar pattern
Intestines : atonic and diarrheal contents
Kidneys : pale in several animals

Necropsy findings by sacrificed animals:
No abnormalities were detected.