acetalization product between glucose and C20-22(even numbered)- alcohol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-11-12 to 2007-02-15

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Principles of method if other than guideline:

no analytical determination of the content of the test item in the tests solutions during the test was made

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): IUPAC name
- Physical state: solid
- Analytical purity: >99%
- Lot/batch No.: T62435
- Expiration date of the lot/batch: 09/06/14
- Stability under test conditions: water 96h
- Storage condition of test material: room temperature 20+-5°C

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 70-80°C
- Boiling point: >300°C
- Vapour pressure: at 25°C 6.66 x 10-5 Pa
- Water solubility (under test conditions): <1mg/L
- log Pow: 9.7
- Stability in water: <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: ready biodegradable

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

all solutions were sterilised before use

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: desmodesmus subspicatus
- Strain: CHODAT
- Source (laboratory, culture collection): laboratory
- Age of inoculum (at test initiation): 24h



ACCLIMATION
- Acclimation period:72h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

After every 24 hours the cell concentration was measured with a spectral photometer

Test conditions

Test temperature:

21-24°C

pH:

8.1-10.1

Nominal and measured concentrations:

1;10;100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): round cuvette
- Material, size, headspace, fill volume: d=6mm
- Initial cells density: 10 000 CFU/ml
- Control end cells density:
- No. of organisms per vessel:10000/ml
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6



GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH:8.1-10.1
- Light intensity and quality:4440-8880 lux



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations:spectrophotometer

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study
- Test concentrations: 1;10;100mg/L
- Results used to determine the conditions for the definitive study:result of Water accommodated Fraction

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 6

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 8

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 9

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Results with reference substance (positive control):

- Results with reference substance valid
- 72h-NOEC=0.1mg/L
-72h-LOEC=0.16mg/L
-72h-ECr50=1.1mg/L
-72h-ECb50=0.36mg/L
-72h-ECy50=0.30mg/L

Any other information on results incl. tables

As the test item is poorly soluble in water, the Water accommodated fraction was tested. This was done by shaking the nominal Load with the appropriiate amount of deionised water for 24 hours , followed by memenrane filtration.

In the main study , three concentrations of the test item in a geometric series (spaced by factor 10, the highest being 100mg/L) were used. The treatments were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours.

The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate, the area under the growth curve (AUC) and the yield could be determined.

The EC50s of potassium dichromate was tested in acurrent reference test. the values lay within the normal range of the laboratory.

The following results for the test item Montanov 202 were determined.

72h NOEC (Growth Rate)= 100 mg/L

72h NOEC (AUC)= 100mg/L

72h NOEC (Yield) = 100mg/L

72h LOEC (Growth Rate)>100 mg/L

72h LOEC (AUC)>100mg/L

72h LOEC (Yield)>100 mg/L

72h ECr50>100mg/L

72h ECb50 >100 mg/L

72h ECy50 >100 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results for the test item Montanov 202 were determined.

72h NOEC (Growth Rate)= 100 mg/L

72h NOEC (AUC)= 100mg/L

72h NOEC (Yield) = 100mg/L


72h LOEC (Growth Rate)>100 mg/L

72h LOEC (AUC)>100mg/L

72h LOEC (Yield)>100 mg/L


72h ECr50>100mg/L

72h ECb50 >100 mg/L

72h ECy50 >100 mg/L

Executive summary:

As the test item is poorly soluble in water, the Water accommodated fraction was tested. This was done by shaking the nominal Load with the appropriate amount of deionised water for 24 hours , followed by memenrane filtration.

In the main study , three concentrations of the test item in a geometric series (spaced by factor 10, the highest being 100mg/L) were used. The treatments were used to incubate the unicellular freshwater green alga Desmodesmus subspicatus for a period of 72 hours.

The cell concentration of each replicate was determined by measuring the absorption of the cuvettes at 440 nm every 24 hours with a spectral photometer. With these measured values, the number of cells was calculated (linear correlation between cell concentration and absorption given). Then the growth rate, the area under the growth curve (AUC) and the yield could be determined.

The EC50s of potassium dichromate was tested in acurrent reference test. the values lay within the normal range of the laboratory.

The following results for the test item Montanov 202 were determined.

72h NOEC (Growth Rate)= 100 mg/L

72h NOEC (AUC)= 100mg/L

72h NOEC (Yield) = 100mg/L

72h LOEC (Growth Rate)>100 mg/L

72h LOEC (AUC)>100mg/L

72h LOEC (Yield)>100 mg/L

72h ECr50>100mg/L

72h ECb50 >100 mg/L

72h ECy50 >100 mg/L