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EC number: 227-369-2 | CAS number: 5809-08-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- There are only limited details in study report, but this is consistent with other reports of this type from this period. Study was done before standardized test guidelines and GLP but was conducted using a standard and documented method at a reputable facility.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 433 draft (Acute Inhalation Toxicity: Fixed Concentration Procedure) (not officially approved)
- Deviations:
- not applicable
- GLP compliance:
- no
- Test type:
- fixed concentration procedure
- Limit test:
- yes
Test material
- Reference substance name:
- 1,1,3,3-tetramethylbutyl hydroperoxide
- EC Number:
- 227-369-2
- EC Name:
- 1,1,3,3-tetramethylbutyl hydroperoxide
- Cas Number:
- 5809-08-5
- Molecular formula:
- C8H18O2
- IUPAC Name:
- 2,4,4-trimethylpentane-2-peroxol
- Test material form:
- other: clear, colourless liquid
- Details on test material:
- The sample, received from the principal on June 13, was a colourless liquid, 95% of which consisted of 2,4,4-trimethylpentyl-2-hydroperoxide. The other components of the tes material were 2,4,4-trimethylpentane-2-ol, diisobutylene and water.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Young rats (Wistar-derived, and reared under S.P.F. conditions) were used for this experiment. The weight of the males varied from 189 to 192 g, that of the females from 133 to 138 g. The animals were obtained from the Central Institute for the Breeding of Laboratory Animals, TNO-Zeist, The Netherlands.
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- not specified
- Remarks:
- Although not stated, exposure was probably whole-body.
- Vehicle:
- air
- Details on inhalation exposure:
- The exposure chamber consists of a horizontally placed glass cylinder (0.90 x 0.15 m) with sampling ports at both ends and contains a perforated stainless steel frame-work for separate accomodation of the rats.
Samples were analysed by means of gas-liquid chromatography, using an Intersmat gas-chromatograph loaded with a SE30 column (0.70 m, ø 1/2 cm), and detected with a flame ionisation detector.
Column-detection and injection temperatures were 75, 85 and 90 °C respectively.
The test compound was gauged by using a 4% solution in acetone. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 480 ppm (2.85 mg/l).
- No. of animals per sex per dose:
- five
- Control animals:
- no
- Details on study design:
- The dynamic atmosphere for the inhalation experiment was formed by passing air (relative humidity 50%) through various columns - airflow per column 1 l/min - containing chromosorb W, AW, saturated with the test substance. Five columns were used in parallel, giving an eventual airfow of 5 l/min (the minimum quantity required to prevent condensation of vapou (H2O)) through the exposure chamber. The temperature inside the cylinder was 24 °C.
A group of five male and five females rats was exposed to an atmosphere saturated with T.M.P.H. for four hours. After the exposure the rats were returned to their living-cages and provided with food and tap water ad libitum during an observation period of 14 days. - Statistics:
- None
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LC0
- Effect level:
- 2.85 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Key result
- Sex:
- female
- Dose descriptor:
- LC0
- Effect level:
- 2.85 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- None.
- Clinical signs:
- other: From the start of the exposure the animals were restless. After half an hour they closed their eyes and had wet noses. The severity of the effects increased during the exposure period. After two hours mouth breathing was observed as well as some incoor
- Body weight:
- No data.
- Gross pathology:
- No data.
- Other findings:
- The intoxication signs observed at 480 ppm, seem to justify the expectation that the 4-hour LC50 will not be very far above this level.
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category III
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- From the results of the present study it can be concluded that the 4-hour LC50 of T.M.P.H. exceeds 480 ppm (2.85 mg/l) but is expected to be less than 10 mg/L.
- Executive summary:
The acute inhalation toxicity of T.M.P.H. was studied in rats, by exposing them to an atmosphere saturated with the test material for a period of four hours.
The dynamic atmosphere for the inhalation experiment was formed by passing air (relative humidity 50%) through various columns - airflow per column 1 l/min - containing chromosorb W, AW, saturated with the test substance. Five columns were used in parallel, giving an eventual airfow of 5 l/min (the minimum quantity required to prevent condensation of vapou (H2O)) through the exposure chamber. The temperature inside the cylinder was 24 °C.
A group of five male and five females rats was exposed to an atmosphere saturated with T.M.P.H. for four hours. After the exposure the rats were returned to their living-cages and provided with food and tap water ad libitum during an observation period of 14 days.
Exposure of the rats to an atmosphere saturated with T.M.P.H. - viz. 480 ppm (2.85 mg/l) under the experimental conditions - caused no mortality. From the start of the exposure the animals were restless. After half an hour they closed their eyes and had wet noses. The severity of the effects increased during the exposure period. After two hours mouth breathing was observed as well as some incoordination of movement. The female rats have been recovered one day after the exposure; the males however, needed about two days before they behaved normally again.
Since no mortality occurred during the subsequent observation period of 14 days, no LD50 value could be determined. From the results of the present study it can be concluded that the 4-hour LC50 of T.M.P.H. exceeds 480 ppm (2.85 mg/l) but is expected to be less than 10 mg/L.
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