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EC number: 260-252-4 | CAS number: 56539-66-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-12-05 to 2002-03-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The Test Guideline includes Part I - the 24h EC 50 acute immobilisation test and Part II - the reproduction test (at least 14 days). Accoding to this study the acute immobilisation test was conducted with a duration of 48 h, part II was not conducted.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test chemical was refrigerated. 500 mg test chemical was diluted in 500 mL dilution water and which was used as 1000 mg/L test solution. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia Magna
-Strain: had been reproduced for 5 years.
- Source: National Institute for Environmental Studies, Ministry of Environment, .
- Age at study initiation (mean and range, SD): Juveniles less than 24 hours old
- Method of breeding: Subculture (maximum rearing period: four weeks, frequency of water exchange: at least twice a week)
- Feeding during test: no feeding
ACCLIMATION
- Acclimation period: December 19, 2001 to January 15, 2002, Parental daphnids were acclimated for 27 days on test condition before testing
- Acclimation conditions (same as test or not): same; water temperature 20+-1°C, 16-8 hours room light-dark photocycle
- Type and amount of food: Chlorella vulgaris, 0.15-0.2 mgC (organic carbon)/organism/day
- Health during acclimation (any mortality observed): death rate of parents was 5% or less in two weeks before exposure, no production of resting eggs or males was observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- no data, artificially prepared water Elendt M4
- Test temperature:
- 20.4 - 20.6°C +/- 1°C
- pH:
- 8.1
- Dissolved oxygen:
- 8 - 8.2 mg/L
- Salinity:
- no data, artificially prepared water Elendt M4
- Nominal and measured concentrations:
- measured concentration was within +/- 20% of the nominal value
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass vessel with screw tubes
- Material, size, headspace, fill volume: 100 mL/container
- Aeration: not described
- No. of organisms per vessel: 5 fleas /container
- No. of vessels per concentration (replicates): 4 containers /concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment,but ph measurement
- Photoperiod: 16-8 hours light dark photocycle
- Light intensity: room light
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Based on the results of the preliminary test, the concentraion of 1000 mg/L and control were set.
- Test concentrations: 1000 mg/L test solution - Reference substance (positive control):
- yes
- Remarks:
- 48 hour median immobilization concentration (EiC50) by potassium dichromate, special grade chemical was 0.67 mg/L (EiC50 value at our site is 0.49 - 1. 1 mg/L, n = 5, since October 1999)
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Infrared absorption spectrum of the refrigerated test chemical was detected at the start and the end of the test, and both spectrums are not contradictory to each other.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- MMB was observed to cause no immobilizing effects on daphnia magna after 48h at a concentration of 1000 mg/L.
- Executive summary:
The test on acute toxicity of 3-methoxy-3-methyl-1-butanol towards invertebrates was performed according to OECD 202. The water flea (Daphnia magna) juveniles, within 24 hours after birth, were used as test organisms. The study was conducted under static conditions over 48h. Each of 20 test organisms was exposed to the test concentration of 1000 mg/L and the control. The concentration of the test substance was within +/- 20% of the nominal value, and each effect concentration was calculated, adopting the nominal value. MMB was found to cause no toxicity on daphnia magna after 24h and after 48h at a concentration of 1000 mg/L . The EC50 24h was > 1000 mg/L and the EC50 48 h was > 1000 mg/L.
Reference
The immobility of the control group under 48 -hour exposure was 0% and
the rate of the fleas that surfaced was 0% and it fulfilled the
conditions for establishing the test.
Mortality or Immobility: No test organism was Immobilized
at any concentration.
-----------------------------------------------------------
Cumulative Number of Dead or Immobilized
Daphnids
Nominal (Percent Mortality or Immobility)
Conc.
-----------------------------------------------------------
mg/L 24 Hour 48 Hour
-----------------------------------------------------------
Control 0 ( 0 ) 0 ( 0 )
1,000 0 ( 0 ) 0 ( 0 )
-----------------------------------------------------------
Description of key information
48h-EC50 > 1000 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
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