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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods
Adequacy of study:
other information
Study period:
2009-06-10 to 2009-08-06

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Total exposure duration:
56 d
Remarks:
Removement of adults after 28 days

Test conditions

Test temperature:
18 - 22.5 deg C
pH:
Application rate
[mg test item/kg soil DW] pH-value
day 0 day 56
Control 5.42 6.13
Solv. Control 5.40 6.40
62.5 5.39 6.18
125 5.39 6.30
250 5.40 6.33
500 5.38 6.07
1000 5.42 6.14
Moisture:
Application rate
[mg test item/kg soil DW] Moisture [%] of dry weight

day 0 day 56
Control 35.6 42.5*
Solv. Control 35.1 45.8*
62.5 34.8 46.7*
125 35.5 46.8*
250 34.9 44.6*
500 35.2 45.2*
1000 34.6 44.6*

*Variation of the water content > 10 %, this deviation is considered to have no impact on the study
Nominal and measured concentrations:
Nominal application rates: 62.5 - 125 - 250 - 500 - 1000 mg test substance/kg soil dry weight (DW)

Results and discussion

Effect concentrations
Duration:
56 d
Effect conc.:
1 000 mg/kg soil dw
Reported statistics and error estimates:
Differences between the control and the solvent control were statistically analysed using t-tests. No statistical differences occurred between the controls concerning biomass and reproduction. Thus, both controls were pooled for further statistical analyses.
One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to
the control. A Normality Test and an Equal Variance Test were conducted prior to running the ANOVA. P-values for both Normality and Equal Variance Test are 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.

Applicant's summary and conclusion