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Ecotoxicological information

Sediment toxicity

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Administrative data

Endpoint:
sediment toxicity: long-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
2008-05-26 to 2008-06-26
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment (October 2007)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
GLP according to German Chemikaliengesetz and Directive 88/320/EEC

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes

Test organisms

Test organisms (species):
Lumbriculus variegatus

Study design

Study type:
laboratory study
Test type:
static
Water media type:
freshwater
Type of sediment:
artificial sediment
Limit test:
yes
Exposure duration
Duration:
28 d
Exposure phase:
total exposure duration

Test conditions

Hardness:
Day 0: Control: > 356 mg/L CaCO3, Solvent Control: 340 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3
Day 28: Control: > 356 mg/L CaCO3, Solvent Control: > 356 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3
Test temperature:
Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 Temperature [°C] 21.1 21.0 20.9
7 Temperature [°C] 21.9 21.2 21.9
14 Temperature [°C] 22.0 20.2 20.2
21 Temperature [°C] 21.8 21.8 21.4
28 Temperature [°C] 21.8 21.2 20.8
pH:
Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW

0 pH 7.74 7.82 7.88
7
pH 7.86 7.86 7.88
14
pH 7.76 7.79 7.86
21
pH 7.58 7.87 8.01
28
pH 8.22 8.27 8.29
Dissolved oxygen:
Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 O2 [mg/L] 6.16 6.78 6.37
7 O2 [mg/L] 6.06 6.05 6.40
14 O2 [mg/L] 6.90 6.22 6.71
21 O2 [mg/L] 7.37 7.11 7.05

28

O2 [mg/L] 7.30 8.04 7.96
Salinity:
Not measured, freshwater conditions
Ammonia:
Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 NH4-N [mg/L] 0.803 0.622 0.797

2 NH4-N [mg/L] 0.114 < 0.02* < 0.02*
5 NH4-N [mg/L] 0.039 0.031 0.113
8 NH4-N [mg/L] 0.045 0.039 0.044
9 NH4-N [mg/L] 0.091 0.050 0.031
12 NH4-N [mg/L] 0.064 0.060 0.068
15 NH4-N [mg/L] 0.089 0.241 0.063
16 NH4-N [mg/L] 1.03 0.715 0.121
19 NH4-N [mg/L] > 2.5* > 2.5* > 2.5*
NH4-N [mg/L] 5.71 5.35 4.29
23 NH4-N [mg/L] 4.84 4.75 5.42
26 NH4-N [mg/L] 6.91 6.01 5.85
28 NH4-N [mg/L] 6.76 6.58 4.30

Nominal and measured concentrations:
ominal concentration limit concentration: 1000 mg test substance/kg sediment DW.
The submission substance has a low water solubility and due to its sorption to particular matter, low volatility and very low biotic and abiotic degradation. It resides predominantly in the solid phase of the sediment. The fraction of the pigment in the overlying water as well as the porewater can be neglected. As no loss of pigment was expected to occur during the exposure period an analytical determination of the pigment in the overlying water and the sediment has been omitted. Based on the fact that the test system consists of 150 mL overlying water 0.008 % pigment would be in the overlying water at saturation and thus 99.992 % remains in the sediment (solids & porewater).
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/kg sediment dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality, behaviour, biomass reproduction
Reported statistics and error estimates:
One Way Analysis of Variance (ANOVA, Dunnett´s Method) was carried out with worm numbers and biomass data for the determination of statistically significant differences compared to the pooled control. Prior to the ANOVA a normality test and an equal variance test were conducted. P-values for both normality and equal variance test were 0.05. The alpha-value (acceptable probability of incorrectly concluding that there is a difference) for ANOVA was alpha = 0.05.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes