Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
28 APR 2005
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Copper (II) sulphate pentahydrate instead of the prescribed 3,5- dichlorophenol as a reference substance. The usage of dichlorophenol is unsuitable for routine lab use.
GLP compliance:
yes (incl. certificate)
Remarks:
GLP according to German "Chemikaliengesetz", EC and OECD Guideline principles

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
21.0 °C
pH:
6.95 (pH of the activated sludge)
Nominal and measured concentrations:
1000 mg/l nominal, no analytical measurements
Reference substance (positive control):
yes
Remarks:
copper (II) sulphate pentahydrate, p.a.

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Reported statistics and error estimates:
The EC50-value of the reference item was calculated with probit analysis using software SIGMAPLOT (Windows) rel. 8.02, 2002, SPSS CORPORATION
Probit values were taken from WEBER (1986).
Confidence intervals were calculated according to a standard procedure (BREITIG & TÜMPLING 1982).

BREITIG, G., TÜMPLING, W. (editors, 1982): Methoden der Wasseruntersuchungen Bd.II. VEB GUSTAV FISCHER VERLAG, Jena.
WEBER, E. (1986): Grundriß der biologischen Statistik, 9. Auflage

Applicant's summary and conclusion

Validity criteria fulfilled:
yes