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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 2000-12-13 to 2001-04-27
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Remarks:
The study was conducted in accordance with "Eco-toxicity test Standards" by Japanese Ministry of the Environment.

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Oryzias latipes

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
No

Test conditions

Hardness:
Total hardness: 63.1 mg/L
Test temperature:
23.8 - 24.0 deg C
pH:
7.4 - 7.8
Dissolved oxygen:
In mg/L:
Control: 6.7 (old medium, 96 hours) to 8.1
Solvent control: 6.5 (old medium, 96 hours) to 8.1
Exposure group (100 mg/L): 5.4 (old medium, 96 hours) to 8.0
Nominal and measured concentrations:
Nominal limit concentration: 100 mg/L;
Measured concentration test start (new medium): 100 mg/L;
Measured concentration at 48 hours (old medium): 99 mg/L;
Geometric mean measured concentration: 100 mg/L.
The concentration of test substance in the control and solvent control were less than the quantification limit (0.06 mg/L).
Reference substance (positive control):
yes
Remarks:
copper (II) sulfate pentahydrate

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks:
& mobility
Reported statistics and error estimates:
No statistical analysis was applied for the calculation of median lethal concentration (LC50), since the study was conducted as a limit test at 100 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes