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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
From 22 MAR 2005 to 24 MAR 2005
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
No test item analysis due to very poor water solubility of test item
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
No test item analysis due to very poor water solubility of test item
GLP compliance:
yes (incl. certificate)
Remarks:
GLP according to German Chemikaliengesetzt and Directive 88/320/EEC

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
no

Test conditions

Hardness:
266 mg CaCO3/L
Test temperature:
20.8 deg C (18-22 deg C, +/- 1 deg C)
pH:
Treatment
Start: 7.72 End: 7.60 - 7.69
Control
Start: 7.8 End: 7.83 - 7.94
Dissolved oxygen:
[mg/L]
Treatment
Start: 8.9 End: 8.09 - 8.14
Control
Start: 8.69 End: 7.67 - 7.93
Nominal and measured concentrations:
Due to the very poor solubility of the test item no test item analysis was carried out.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate p.a. (Merck) 100% purity, Batch No. K 28974764

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
EC10- and EC-50-values were only calculated for the reference analysis item via probit according to WEBER (1986).
Calculation of the confidence intervals were carried out using standard procedures according to BREITIG & TÜMPLING (1982).

BREITIG G. & TÜMPLING W. (1982): Methoden der Wasseruntersuchungen Bd.II, VEB Gustav Fischer Verlag, Jena.
WEBER E. (1986): Grundriss der biologischen Statistik, 9. Auflage

Applicant's summary and conclusion

Validity criteria fulfilled:
yes