Chlorodiphenylphosphine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Chlorodiphenylphosphine

EC Number:

214-093-2

EC Name:

Chlorodiphenylphosphine

Cas Number:

1079-66-9

Molecular formula:

C12H10ClP

IUPAC Name:

diphenylphosphinous chloride

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start (t=0 h) and the end of the test (t=48 h)
Samples will be taken from all concentrations and the control

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A loading rate of 100 mg CHLORODIPHENYLPHOSPHINE per litre of test medium will be prepared and magnetically stirred for one day. The water saturated solution (SS) will be taken out by siphoning and used as the highest test concentration. Lower test concentrations will be
prepared by proportional dilution of the SS in test medium.
The tests will be carried out without adjustment of the pH, except if pH values of test solutions are outside the optimal pH range for the species to be tested. No correction will be made for the purity/composition of the test item.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name, Strain/clone: Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
- Source:In-house laboratory culture with a known history.
- Age of parental stock (mean and range, SD): For the test selection of young daphnia with an age of < 24 hours, from parental daphnids of more than two weeks old.
- Feeding during test
- Food type: No

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

The hardness: approximately 180 mg/L expressed as CaCO3

Test temperature:

18-22°C, constant within ±1°C.

pH:

Between 6 and 9. Should normally not vary by more than 1.5
unit.

Dissolved oxygen:

≥ 3 mg/L at the end of the test.

Salinity:

Not applicable, Freshwater

Details on test conditions:

TEST SYSTEM
- Test vessel: All-glass, with sufficient volume to accommodate the organisms. In case of volatile items test vessels will be closed air-tight and the headspace will be reduced to a minimum.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
Adjusted ISO medium

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours photoperiod daily; should the test item be light sensitive, the test will be performed in the dark.
- Temperature: 18-22°C, constant within ±1°C.
- Aeration: no aeration of the test solution

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h-EC50 for Daphnia magna exposed to the degradation product of CHLORODIPHENYLPHOSPHINE was 17 mg/L based on initial and stable exposure concentrations (95% confidence interval between 14 and 21 mg/L).

Executive summary:

The 48h-EC50 for Daphnia magna exposed to the degradation product of CHLORODIPHENYLPHOSPHINE was 17 mg/L based on initial and stable exposure concentrations (95% confidence interval between 14 and 21 mg/L).