Trifluoro(tetrahydrofuran)boron

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes (incl. QA statement)

Remarks:

testing lab.

Test material

Test material information

Constituent 1

Reference substance name:

Trifluoro(tetrahydrofuran)boron

EC Number:

207-325-9

EC Name:

Trifluoro(tetrahydrofuran)boron

Cas Number:

462-34-0

Molecular formula:

C4H8BF3O

IUPAC Name:

trifluoro(oxolan-1-ium-1-yl)boranuide

Details on test material:

Name of the test substance used in the study report: Borfluorid-Tetrahydrofuran
Lot No. 5/93

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

Young adult rabbits were used. They were singly housed in stainless wire mesh cages (no bedding) in fully air-conditioned rooms with a temperature of 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 hours dark and 12 hours light. The animals were offered a standardized rabbit laboratory diet (130 g/animal/day) as well as tap water (ca. 130 g/animal/day).
The acclimatization period was at least 1 week. Body weight determination was done shortly before application of the test substance.

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: the fur was removed by clipping the dorsal of the trunk of the animal

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of the same animal

Amount / concentration applied:

0.5 ml

Duration of treatment / exposure:

3 minutes, 1 hour

Observation period:

3-min exposure: 72 hours
1-hour exposure: 24 hours

Number of animals:

3 (3-min exposure); 1 (1-h exposure)

Details on study design:

Application site: upper third of the back or flanks
Application volume: The test patches (2.5 x 2.5 cm) were moistened with 0.5 ml of the liquid test substance.
Readings were done 1 h, 24 h, 48 h and 72 h after removal of the test patches.
A check for general observations and for any dead or moribund animal(s) was made twice each working day and once on weekendes and public holidays.
Additional investigation: Because the visual assessment of the skin did not allow a statement with respect to the depth of necrotic changes, a cross incision of the trated skin has been performed after the rabbit was killed at study termination.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

0.2

Max. score:

4

Reversibility:

fully reversible within: 72 hours

Remarks on result:

other: 3-min application

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 - 48 h

Score:

0

Max. score:

4

Remarks on result:

other: 3-min application

Results 3

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 24 h

Score:

4

Max. score:

4

Reversibility:

other: not determined due to study termination after 24 h

Irritant / corrosive response data:

1-hour application: The average score for irritation could not be calculated because of visual necrosis and therefrom resulting study termination 24 hours after removal of the test patch.

Other effects:

The necrotic change of the skin was assessed by macroscopic pathology indicating full-thickness necrosis.

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information